Elfin is expressed during early heart development

Elfin (previously named CLIM1) is a protein that possesses an N‐terminal PDZ domain and a C‐terminal LIM domain. It belongs to the family of Enigma proteins. Enigma proteins are a family of cytoplasmic proteins that contain an N‐terminal PDZ domain and a series of C‐terminal LIM domains. By virtue o...

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Bibliographic Details
Published in:Journal of cellular biochemistry 2001, Vol.83 (3), p.463-472
Main Authors: Kotaka, Masayo, Lau, Yee-man, Cheung, Kwok-kuen, Lee, Simon M.Y., Li, Hoi-yeung, Chan, Wood-yee, Fung, Kwok-pui, Lee, Cheuk-yu, Waye, Mary M.Y., Tsui, Stephen K.W.
Format: Article
Language:English
Subjects:
Actinin - metabolism
Amino Acid Sequence
Animals
Cell Line
CLIM1
CLP-36
Cytoplasm - metabolism
Cytoskeleton - metabolism
DNA, Complementary - metabolism
embryonic development
Enigma protein
Gene Expression Regulation, Developmental
Green Fluorescent Proteins
Heart - embryology
heart muscle
In Situ Hybridization
LIM domain
LIM Domain Proteins
Luminescent Proteins - metabolism
Mice
Molecular Sequence Data
Myocardium - metabolism
PDZ domain
Protein Binding
Protein Structure, Tertiary
RNA, Messenger - metabolism
Sequence Homology, Amino Acid
Stress Fibers - metabolism
Time Factors
Tissue Distribution
Transcription Factors - biosynthesis
Transcription Factors - chemistry
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Summary:Elfin (previously named CLIM1) is a protein that possesses an N‐terminal PDZ domain and a C‐terminal LIM domain. It belongs to the family of Enigma proteins. Enigma proteins are a family of cytoplasmic proteins that contain an N‐terminal PDZ domain and a series of C‐terminal LIM domains. By virtue of these two protein interacting domains, Enigma proteins are capable of protein–protein interactions. It has been proposed that Enigma proteins may act as adapters between kinases and the cytoskeleton. We have previously shown that Elfin is most abundantly expressed in the heart and it colocalizes with α‐actinin 2 at the Z‐disks of the myocardium. In this report, Elfin was shown to localize at the actin stress fibers of myoblasts, as revealed by green fluorescent protein (GFP) tagging. In situ hybridization and immunostaining showed that Elfin expression begins at an early stage in mouse development and is present throughout the developing heart. Taken together, our experimental results suggest that Elfin may play an important role in myofibrillogenesis and heart development. J. Cell. Biochem. 83: 463–472, 2001. © 2001 Wiley‐Liss, Inc.
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.1244