Increased epithelial cell proliferation in very premature baboons with chronic lung disease

Division of Neonatology, Strong Children's Research Center, Department of Pediatrics, University of Rochester, Rochester, New York 14642 Coordinated proliferation of lung cells is required for normal lung growth and differentiation. Chronic injury to developing lung may disrupt normal patterns...

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Bibliographic Details
Published in:American journal of physiology. Lung cellular and molecular physiology 2002-11, Vol.283 (5), p.991-L1001
Main Authors: Maniscalco, William M, Watkins, Richard H, O'Reilly, Michael A, Shea, Colleen P
Format: Article
Language:English
Subjects:
Animals
Bronchopulmonary Dysplasia - mortality
Bronchopulmonary Dysplasia - pathology
Cell Differentiation
Death
Disease Models, Animal
Embryonic and Fetal Development
Female
Gestational Age
Humans
Infant, Newborn
Lung - embryology
Lung - pathology
Papio
Pregnancy
Respiratory Mucosa - growth & development
Respiratory Mucosa - pathology
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Summary:Division of Neonatology, Strong Children's Research Center, Department of Pediatrics, University of Rochester, Rochester, New York 14642 Coordinated proliferation of lung cells is required for normal lung growth and differentiation. Chronic injury to developing lung may disrupt normal patterns of cell proliferation. To examine patterns of cell proliferation in injured developing lungs, we investigated premature baboons delivered at 125 days gestation (~67% of term) and treated with oxygen and ventilation for 6,   14, or 21 days (PRN). Each PRN treatment group contained 3 or 4 animals. During normal in utero lung development, the proportion of proliferating lung cells declined as measured by the cell-cycle marker Ki67. In the PRN group, the proportion of proliferating lung cells was 2.5-8.5-fold greater than in corresponding gestational controls. By 14 days of treatment, the proportion of cells that expressed pro-surfactant protein B (proSP-B) was ~2.5-fold greater than in gestational controls. In the PRN group, 41% of proliferating cells expressed proSP-B compared with 5.8% in the gestational controls. By 21 days of treatment, proliferation of proSP-B-expressing epithelial cells declined substantially, but the proportion of proliferating non-proSP-B-expressing cells increased approximately sevenfold. These data show that the development of chronic lung disease is associated with major alterations in normal patterns of lung-cell proliferation. development; oxygen; differentiation; bronchopulmonary dysplasia
ISSN:1040-0605
1522-1504
DOI:10.1152/ajplung.00050.2002