Polystyrene derivatives as candidates for extracorporeal adsorption of Factor VIII antibodies in the management of haemophiliac patients

Background and Objectives This article presents a new approach for removing the Factor VIII inhibitors (anti‐FVIII) in haemophiliac patients by immunoadsorption using an affinity matrix. Materials and Methods Ten blood samples from haemophiliac patients with anti‐FVIII were assayed for antibodies, t...

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Bibliographic Details
Published in:Vox sanguinis 2002-10, Vol.83 (3), p.214-221
Main Authors: Boisson-Vidal, C., Haouam, M., Lakhiari, H., Huguet, H., Dahri, L., Sultan, Y., Jozefonvicz, J., Siali, R.
Format: Article
Language:English
Subjects:
affinity chromatography
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
anti-FVIII inhibitors
Autoantibodies - blood
Autoantibodies - isolation & purification
Biological and medical sciences
Blood Coagulation Factors - analysis
Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis
Chromatography, Affinity
coagulation factors
Complement C3 - analysis
Disease Management
Factor VIII - immunology
functionalized polystyrene
haemophiliac plasma purification
Hemophilia A - immunology
Hemophilia A - therapy
Humans
Immunoglobulins - blood
Immunosorbent Techniques
Medical sciences
Polystyrenes
Resins, Synthetic - standards
Transfusions. Complications. Transfusion reactions. Cell and gene therapy
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Summary:Background and Objectives This article presents a new approach for removing the Factor VIII inhibitors (anti‐FVIII) in haemophiliac patients by immunoadsorption using an affinity matrix. Materials and Methods Ten blood samples from haemophiliac patients with anti‐FVIII were assayed for antibodies, total immunoglobulins, procoagulant proteins and complement C3 protein after circulation over one or two columns filled with the polymers under investigation. Results These new synthetic sorbents are able to remove in vitro 90% of anti‐FVIII from haemophiliac plasma with inhibitors (up to 540 Bethesda Units/ml). Neither coagulation factor adsorption nor effects on complement system activation were observed. Conclusions The data presented clearly show that these polymers allow a rapid and efficient reduction of inhibitor titre. In view of the parameters studied, these polymers fulfil the requirements for use in a blood purification process to decrease high inhibitor titres without losing essential proteins.
ISSN:0042-9007
1423-0410
DOI:10.1046/j.1423-0410.2002.00190.x