Mouse novel Ly9: a new member of the expanding CD150 (SLAM) family of leukocyte cell-surface receptors

Human CS1, also known as novel Ly9, 19A24, or CRACC, is a member of the immunoglobulin gene superfamily (IgSF) expressed on natural killer cells and other leukocytes. Here we describe the cloning of the mouse homologue of this gene. The mouse novel Ly9 gene is shown to encode a transmembrane protein...

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Bibliographic Details
Published in:Immunogenetics (New York) 2002-09, Vol.54 (6), p.394-402
Main Authors: Tovar, Victoria, del Valle, Juana, Zapater, Nuria, Martin, Margarita, Romero, Xavier, Pizcueta, Pilar, Bosch, Jaime, Terhorst, Cox, Engel, Pablo
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antigens, CD - genetics
Antigens, CD - metabolism
Base Sequence
Carrier Proteins - metabolism
Chromosome Mapping
Cloning, Molecular
DNA, Complementary - genetics
Female
Gene Expression
Glycoproteins - genetics
Glycoproteins - metabolism
Humans
Immunoglobulins - genetics
Immunoglobulins - metabolism
Intracellular Signaling Peptides and Proteins
Leukocytes - immunology
Male
Mice
Molecular Sequence Data
Pregnancy
Receptors, Cell Surface
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sequence Homology, Amino Acid
Signaling Lymphocytic Activation Molecule Associated Protein
Signaling Lymphocytic Activation Molecule Family
Signaling Lymphocytic Activation Molecule Family Member 1
Species Specificity
Tissue Distribution
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Summary:Human CS1, also known as novel Ly9, 19A24, or CRACC, is a member of the immunoglobulin gene superfamily (IgSF) expressed on natural killer cells and other leukocytes. Here we describe the cloning of the mouse homologue of this gene. The mouse novel Ly9 gene is shown to encode a transmembrane protein composed of two extracellular immunoglobulin-like domains, a transmembrane region and an 88-amino acid cytoplasmic domain. Mouse novel Ly9 is structurally similar to the extracellular domains of CD84 and CD229 (Ly9). Both mouse and human novel Ly9 genes mapped close to the CD229gene in a region where other members of the CD150 family have also been mapped, and analysis of their genomic sequences showed that they have an identical intron/exon organization. Northern blot analysis revealed that the expression of mouse and human novel Ly9 was predominantly restricted to hematopoietic tissues, with the exception of testis. Here we show that SAP (SH2D1A), an adapter protein responsible for the X-linked lymphoproliferative disease, binds to the phosphorylated cytoplasmic tail of human but not mouse novel Ly9. Taken together, these data indicate that mouse novel Ly9 is a new member of the expanding CD150 family of cell surface receptors.
ISSN:0093-7711
1432-1211
DOI:10.1007/s00251-002-0483-3