Dominance of virus-specific CD8 T cells in human primary cytomegalovirus infection

Cellular immune responses are of high importance in initiating and maintaining immunity against virus infections. Whereas the cellular immune response during persistent cytomegalovirus (CMV) infection is well assessable, the individual contribution of CD4 and CD8 T cell responses during primary infe...

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Bibliographic Details
Published in:Journal of the American Society of Nephrology 2002-10, Vol.13 (10), p.2577-2584
Main Authors: SESTER, Martina, SESTER, Urban, GÄRTNER, Barbara C, GIRNDT, Matthias, MEYERHANS, Andreas, KÖHLER, Hans
Format: Article
Language:English
Subjects:
Adult
Aged
Antibody Formation
Biological and medical sciences
CD4-Positive T-Lymphocytes - immunology
CD4-Positive T-Lymphocytes - pathology
CD8-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - pathology
Cell Count
Combined surgery. Multiple transplantations
Cytomegalovirus - immunology
Cytomegalovirus Infections - drug therapy
Cytomegalovirus Infections - etiology
Cytomegalovirus Infections - immunology
Cytomegalovirus Infections - pathology
Cytomegalovirus Infections - virology
Ganciclovir - therapeutic use
Humans
Immunoglobulin G - blood
Immunoglobulin M - blood
Kidney Transplantation - adverse effects
Male
Medical sciences
Middle Aged
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Viral Load
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Summary:Cellular immune responses are of high importance in initiating and maintaining immunity against virus infections. Whereas the cellular immune response during persistent cytomegalovirus (CMV) infection is well assessable, the individual contribution of CD4 and CD8 T cell responses during primary infection has not been described. A novel whole-blood assay, which relies on the flow-cytometric detection of antigen-induced cytokine expression, was used to characterize CMV-specific CD4 and CD8 T cell responses during primary infection of CMV seronegative recipients of a renal allograft from a CMV seropositive donor. These T cell responses were compared with long-term CMV-positive patients with known history of transplantation-related seroconversion. Results were further correlated to CMV load and serum IgG and IgM. The long-term seroconverted patients consistently showed a dominant CMV-specific CD4 T cell response (median frequencies: CD4, 1.12% [range, 0.35 to 8.10%] versus CD8 0.13% [range,
ISSN:1046-6673
1533-3450
DOI:10.1097/01.ASN.0000030141.41726.52