Detection of Infectious Immune Complexes in Human Immunodeficiency Virus Type 1 (DIV-l) Infections: Correlation with Plasma Viremia and CD4 Cell Counts

The detection of infectious immune complexes in plasma after human immunodeficiency virus (HIV) infection may be useful as a surrogate marker of progression of disease and may help in understanding the pathogenesis of AIDS. Polyethylene glycol (PEG) precipitates of plasma were tested for the presenc...

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Bibliographic Details
Published in:The Journal of infectious diseases 1991-10, Vol.164 (4), p.765-769
Main Authors: Fiscus, Susan A., Wallmark, Ewa B., Folds, J. D., Fryer, J., van der Horst, C. M.
Format: Article
Language:English
Subjects:
Acquired Immunodeficiency Syndrome - diagnosis
Acquired Immunodeficiency Syndrome - immunology
AIDS
AIDS related complex
AIDS-Related Complex - diagnosis
AIDS-Related Complex - immunology
AIDS/HIV
Antigen antibody complex
Antigen-Antibody Complex - blood
Antigens
Biological and medical sciences
Biological markers
Blood plasma
CD4-Positive T-Lymphocytes
Cell culture techniques
Chemical Precipitation
Concise Communications
Cultured cells
Gene Products, gag - analysis
HIV
HIV Antigens - analysis
HIV Core Protein p24
HIV Infections - diagnosis
HIV Infections - immunology
HIV-1 - immunology
Humans
Infectious diseases
Leukocyte Count
Medical sciences
Polyethylene Glycols
Viral Core Proteins - analysis
Viral diseases
Viremia
Viremia - diagnosis
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Summary:The detection of infectious immune complexes in plasma after human immunodeficiency virus (HIV) infection may be useful as a surrogate marker of progression of disease and may help in understanding the pathogenesis of AIDS. Polyethylene glycol (PEG) precipitates of plasma were tested for the presence ofHIV p24 antigen and infectious virus. Results were compared with data from cell and plasma cultures, plasma p24 antigen, CD4 cell counts, and stage of disease. PEG precipitation increased the detection rate of the p24 antigen assay from 38.3% to 58.7%. There was a significant correlation between precipitable p24 antigen and plasma viremia, changes in CD4 cell counts, and progression of disease. The sensitivity of the PEG-precipitable p24 antigen assay versus traditional p24 antigen testing was 59.0% and the specificity 91.7%. The assay was reproducible and may be a useful determinant of viral load, clinical progression, and antiretroviral efficacy.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/164.4.765