Effects of a time varying strong magnetic field on release of cytosolic free Ca2+ from intracellular stores in cultured bovine adrenal chromaffin cells

This study was made to explain the mechanisms for the effects of exposure to a time varying 1.51 T magnetic field on the intracellular Ca2+ signaling pathway. The exposure inhibited an increase in intracellular Ca2+ concentration ([Ca2+]i) in bovine chromaffin cells induced by addition of bradykinin...

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Published in:Bioelectromagnetics 2002-10, Vol.23 (7), p.505-515
Main Authors: Ikehara, Toshitaka, Park, Ki Ho, Yamaguchi, Hisao, Hosokawa, Keiko, Houchi, Hitoshi, Azuma, Mami, Minakuchi, Kazuo, Kashimoto, Hideki, Kitamura, Mitsuo, Kinouchi, Yohsuke, Yoshizaki, Kazuo, Miyamoto, Hiroshi
Format: Article
Language:English
Subjects:
5-trisphosphate
Adrenal Glands - metabolism
Animals
Biological Transport
bradykinin
Bradykinin - pharmacology
Ca2+ release
Calcium - metabolism
Cattle
Cells
Chromaffin Cells - metabolism
Chromaffin Cells - radiation effects
eddy current
Electromagnetic Fields
inositol 1
Inositol 1,4,5-Trisphosphate - metabolism
inositol 1,4,5‐trisphosphate
IP3 receptor
Signal Transduction
Time Factors
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Summary:This study was made to explain the mechanisms for the effects of exposure to a time varying 1.51 T magnetic field on the intracellular Ca2+ signaling pathway. The exposure inhibited an increase in intracellular Ca2+ concentration ([Ca2+]i) in bovine chromaffin cells induced by addition of bradykinin (BK) to a Ca2+ free medium. The exposure did not change BK induced production of inositol 1,4,5‐trisphosphate (IP3). [Ca2+]i was markedly increased in IP3 loaded cells, and this increase was inhibited by the magnetic field exposure. A similar increase in [Ca2+]i by other drugs, which stimulated Ca2+ release from intracellular Ca2+ stores, was again inhibited by the same exposure. However, transmembrane Ca2+ fluxes caused in the presence of thapsigargin were not inhibited by the magnetic field exposure in a Ca2+ containing medium. Inhibition of the BK induced increase in [Ca2+]i by the exposure for 30 min was mostly recovered 1 h after exposure ended. Our results reveal that the magnetic field exposure inhibits Ca2+ release from intracellular Ca2+ stores, but that BK bindings to BK receptors of the cell membrane and intracellular inositol IP3 production are not influenced. Bioelectromagnetics 23:505–515, 2002. © 2002 Wiley‐Liss, Inc.
ISSN:0197-8462
1521-186X
DOI:10.1002/bem.10043