Application of a nuclease from rye nucleus for structural studies of plant ribonucleic acids

A new nuclease (Rn) isolated from rye nucleus was applied for the structural studies of methionine initiator transfer ribonucleic acid and ribosomal 5S rRNA from yellow lupin seeds. The enzyme shows high specificity for some regions of both RNAs. The dihydrouridine and ribothymidine loops which are...

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Bibliographic Details
Published in:Phytochemistry (Oxford) 1991, Vol.30 (6), p.1749-1752
Main Authors: Przykorska, A., Nalaskowska, M., Kuligowska, E., Szarkowski, J.W., Barciszewski, J.
Format: Article
Language:English
Subjects:
5S rRNA
Analytical, structural and metabolic biochemistry
Base Sequence
Biological and medical sciences
biosynthesis
Biotechnology
Cell Nucleus - chemistry
chemical structure
Fundamental and applied biological sciences. Psychology
genetic code
Gramineae
Lupinus
methionine
methionine initiator tRNA
molecular conformation
Molecular Sequence Data
nucleases
nuclei
Nucleic Acid Conformation
Nucleic acids
plant tRNA
Ribonucleases - isolation & purification
Ribonucleases - metabolism
ribosomal RNA
RNA - chemistry
RNA - metabolism
RNA, Double-Stranded - metabolism
Rna, ribonucleoproteins
RNA, Ribosomal, 5S - metabolism
RNA, Transfer, Met - metabolism
rye
Secale - analysis
Secale cereale
seeds
tertiary structure of tRNA and 5S rRNA
transfer RNA
tRNA iMet
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Summary:A new nuclease (Rn) isolated from rye nucleus was applied for the structural studies of methionine initiator transfer ribonucleic acid and ribosomal 5S rRNA from yellow lupin seeds. The enzyme shows high specificity for some regions of both RNAs. The dihydrouridine and ribothymidine loops which are supposed to be involved in the tertiary interactions of the methionine initiator tRNA were hydrolysed. The anticodon loop is not digested at all. 5S rRNA was digested in single stranded regions (loops). The cleavage pattern of the tRNA and 5S rRNA obtained with Rn enzyme, suggests not only the high specificity toward single stranded regions, but also some dependence on their tertiary structure.
ISSN:0031-9422
1873-3700
DOI:10.1016/0031-9422(91)85005-K