Selective Inactivation of Human Neutrophil Elastase by Synthetic Tannin

Tannins of natural or synthetic origin are well-known adjuvants in topical anti-inflammatory therapy of skin diseases. In this study, the influence of synthetic tannin on neutrophil accumulation, enzyme release, and on the proinflammatory activity of neutrophil-derived enzymes was investigated. The...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of investigative dermatology 1991-09, Vol.97 (3), p.529-533
Main Authors:	Mrowietz, Ulrich, Ternowitz, Thomas, Wiedow, Oliver
Format:	Article
Language:	eng
Subjects:	Adjuvants Analytical, structural and metabolic biochemistry Antiinflammatory agents beta -Glucuronidase Biological and medical sciences Cathepsin G Cell Degranulation - drug effects Chemotaxis Chemotaxis, Leukocyte - drug effects Chymotrypsin - metabolism Complement C3b - pharmacology Degranulation Dermatology Elastase Enzyme Activation - drug effects Enzymes Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Glucuronidase - metabolism Humans Hydrolases Inflammation Leukocyte elastase Leukocytes (neutrophilic) N-Formylmethionine Leucyl-Phenylalanine - pharmacology Neutrophils - cytology Neutrophils - enzymology Opsonization Pancreatic Elastase - blood Peroxidase Serine proteinase Skin diseases Tannic acid Tannins - pharmacology Zymosan - pharmacology
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Tannins of natural or synthetic origin are well-known adjuvants in topical anti-inflammatory therapy of skin diseases. In this study, the influence of synthetic tannin on neutrophil accumulation, enzyme release, and on the proinflammatory activity of neutrophil-derived enzymes was investigated. The results show that synthetic tannin (Tamol) specifically inhibits the neutrophil serine protease human leukocyte elastase (HLE) in an irreversible manner with a half-maximal inhibitory concentration (IC50) of 0.3 μg/ml. Exogenous protein partially abolished the tannin-dependent HLE inhibition (IC50 of Tamol at 1% protein-concentration:1.0 μg/ml). Synthetic tannin did not influence the activities of other neutrophil enzymes like Cathepsin G, β-glucuronidase, and myeloperoxidase. The specificity of Tamol for HLE was further substantiated by the lack of inhibition of other serine proteases. Additionally, Tamol had no effect on f-met-leuphe–induced neutrophil chemotaxis and did not alter enzyme degranulation of neutrophils in response to f-met-leuphe and opsonized zymosan. We conclude from our results that the anti-inflammatory properties of synthetic tannin may at least in part be due to inactivation of the proinflammatory protease HLE.
ISSN:	0022-202X 1523-1747