Differing expression of enzymes of the glyoxalase system in superficial and invasive bladder carcinomas

This work aimed to study the activities of the glyoxalase system enzymes (glyoxalase I (GI) and glyoxalase II (GII) and their gene expression in human bladder carcinomas compared with the corresponding normal mucosa. Samples of these tissues were collected from 26 patients with superficial (SBC) or...

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Bibliographic Details
Published in:European journal of cancer (1990) 2002-09, Vol.38 (14), p.1946-1950
Main Authors: Mearini, E, Romani, R, Mearini, L, Antognelli, C, Zucchi, A, Baroni, T, Porena, M, Talesa, V.N
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Biological and medical sciences
Bladder cancer
Blotting, Northern
Electrophoresis, Gel, Two-Dimensional - methods
Female
Gene Expression
Glyoxalase system
Humans
Lactoylglutathione Lyase - genetics
Lactoylglutathione Lyase - metabolism
Male
Medical sciences
Methylglyoxal
Middle Aged
Neoplasm Invasiveness
Neoplasm Proteins - genetics
Neoplasm Proteins - metabolism
Nephrology. Urinary tract diseases
Thiolester Hydrolases - genetics
Thiolester Hydrolases - metabolism
Tumors of the urinary system
Urinary Bladder Neoplasms - enzymology
Urinary tract. Prostate gland
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Summary:This work aimed to study the activities of the glyoxalase system enzymes (glyoxalase I (GI) and glyoxalase II (GII) and their gene expression in human bladder carcinomas compared with the corresponding normal mucosa. Samples of these tissues were collected from 26 patients with superficial (SBC) or invasive bladder cancer (IBC) and used to evaluate enzyme activity and gene expression by northern blot analysis. In keeping with the electrophoretic pattern and the expression level of the respective genes, GI activity significantly increased in SBC samples, while it remained unchanged in IBC samples compared with the normal mucosa. In contrast, GII showed a higher activity in the tumour (either SBC or IBC samples) versus normal tissues. These results confirm the role of the glyoxalases in detoxifying cytotoxic methylglyoxal (MG) in bladder cancer. The differing levels of GI activity level and gene expression of GI between the SBC and IBC samples could help in their differential diagnosis.
ISSN:0959-8049
1879-0852
DOI:10.1016/S0959-8049(02)00236-8