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Molecular cloning of gp49, a cell-surface antigen that is preferentially expressed by mouse mast cell progenitors and is a new member of the immunoglobulin superfamily
gp49 is a Mr 49,000 glycoprotein expressed on the surface of mouse bone marrow-derived mast cells, which are progenitors for the major in vivo mast cell subclasses, typified by intestinal mucosal mast cells and serosal mast cells. The amino-terminal amino acid sequence of gp49 was determined after i...
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Published in: | The Journal of biological chemistry 1991-08, Vol.266 (24), p.15966-15973 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | gp49 is a Mr 49,000 glycoprotein expressed on the surface of mouse bone marrow-derived mast cells, which are progenitors for
the major in vivo mast cell subclasses, typified by intestinal mucosal mast cells and serosal mast cells. The amino-terminal
amino acid sequence of gp49 was determined after isolation of the solubilized membrane protein by affinity chromatography
with the B23.1 anti-gp49 monoclonal antibody. Redundant oligonucleotides were used to isolate a full-length 1.3-kilobase cDNA
from a mouse mast cell library. The predicted amino acid sequence contains a signal peptide of 23 residues, an extracellular
domain of 215 residues with three potential sites of N-linked glycosylation, a transmembrane domain of 23 residues, and a
cytoplasmic tail of 42 residues. Hybridization of the gp49 cDNA was limited to mRNA extracted from those cell types that also
bound the B23.1 monoclonal antibody as assessed by cytofluorographic analyses. The predicted extracellular domain of gp49
contains two regions of 48 and 51 amino acids, each flanked by cysteine residues. Both regions meet criteria for being C2-type
domains of the immunoglobulin superfamily based upon the alignment of consensus amino acids and their predicted secondary
structure organization. Thus, gp49, a membrane glycoprotein preferentially expressed by the progenitor mast cell population,
is a new member of the immunoglobulin superfamily. |
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ISSN: | 0021-9258 1083-351X |
DOI: | 10.1016/S0021-9258(18)98502-9 |