Pro-Leu-Ser/Thr-Pro is a consensus primary sequence for substrate protein phosphorylation. Characterization of the phosphorylation of c-myc and c-jun proteins by an epidermal growth factor receptor threonine 669 protein kinase

A growth factor-stimulated (MAP2-related) protein kinase, ERT, that phosphorylates the epidermal growth factor receptor at Thr669 has been purified from KB human tumor cells by Northwood and co-workers (Northwood, I. C., Gonzalez, F. A., Wartmann, M., Raden, D. L., and Davis, R. J. (1991) J. Biol. C...

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Bibliographic Details
Published in:	The Journal of biological chemistry 1991-08, Vol.266 (23), p.15277-15285
Main Authors:	Alvarez, E, Northwood, I C, Gonzalez, F A, Latour, D A, Seth, A, Abate, C, Curran, T, Davis, R J
Format:	Article
Language:	eng
Subjects:	Amino Acid Sequence Animals Chickens DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism epidermal growth factor ErbB Receptors - genetics ErbB Receptors - metabolism Humans Jun protein Mice Molecular Sequence Data Myc protein Peptide Mapping Phosphorylation Protein-Tyrosine Kinases - genetics Protein-Tyrosine Kinases - metabolism Proto-Oncogene Proteins c-jun Proto-Oncogene Proteins c-myc - genetics Proto-Oncogene Proteins c-myc - metabolism Rats Sequence Alignment Substrate Specificity Transcription Factors - genetics Transcription Factors - metabolism
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Summary:	A growth factor-stimulated (MAP2-related) protein kinase, ERT, that phosphorylates the epidermal growth factor receptor at Thr669 has been purified from KB human tumor cells by Northwood and co-workers (Northwood, I. C., Gonzalez, F. A., Wartmann, M., Raden, D. L., and Davis, R. J. (1991) J. Biol. Chem. 266, 15266-15276). The ERT protein kinase has a restricted substrate specificity, and the structural determinants employed for substrate recognition by this enzyme have not been defined. As an approach toward understanding the specificity of substrate phosphorylation, we have used an in vitro assay to identify additional substrates for the ERT protein kinase. In this report we describe two novel substrates: (a) the human c-myc protein at Ser62 and (b) the rat c-jun protein at Ser246. Alignment of the primary sequences surrounding the phosphorylation sites located within the epidermal growth factor receptor (Thr669), Myc (Ser62), and Jun (Ser246) demonstrated a marked similarity. The observed consensus sequence was Pro-Leu-Ser/Thr-Pro. We propose that this sequence forms part of a substrate structure that is recognized by the ERT protein kinase.
ISSN:	0021-9258 1083-351X