Evaluation of methods for the identification of Salmonella enterica serotype Typhimurium DT104 from poultry environmental samples

An increase in the prevalence of Salmonella enterica serotype Typhimurium DT104 has been reported worldwide. This study examined the prevalence of this microorganism in poultry environmental samples from commercial layer flocks and pullet environments as well as the sensitivity and specificity of a...

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Bibliographic Details
Published in:Journal of microbiological methods 2004-07, Vol.58 (1), p.79-86
Main Authors: Leon-Velarde, Carlos G, Cai, Hugh Y, Larkin, Carolyn, Bell-Rogers, Patricia, Stevens, R.William C, Odumeru, Joseph A
Format: Article
Language:English
Subjects:
Animals
Antimicrobial resistance
Bacteriological methods and techniques used in bacteriology
Bacteriology
Bacteriophage Typing - veterinary
Biological and medical sciences
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
Drug Resistance, Multiple, Bacterial
Environmental samples
Female
Fundamental and applied biological sciences. Psychology
Housing, Animal
Microbiology
Ontario - epidemiology
PCR
Polymerase Chain Reaction - veterinary
Poultry
Poultry Diseases - epidemiology
Poultry Diseases - microbiology
Prevalence
Salmonella enterica
Salmonella Infections, Animal - epidemiology
Salmonella Infections, Animal - microbiology
Salmonella typhimurium - genetics
Salmonella typhimurium - isolation & purification
Salmonella Typhimurium DT104
Sensitivity and Specificity
Serotyping - veterinary
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Summary:An increase in the prevalence of Salmonella enterica serotype Typhimurium DT104 has been reported worldwide. This study examined the prevalence of this microorganism in poultry environmental samples from commercial layer flocks and pullet environments as well as the sensitivity and specificity of a PCR-based method, and multiple antibiotic resistance profile of Salmonella serogroup B isolates in relation to the serotype and phagetype reference method for the identification of Salmonella Typhimurium DT104. A total of 435 Salmonella isolates were obtained from poultry house environmental samples tested during a 20-month period representing a prevalence of 5.5%. Of these, 313 (72%) isolates were identified as Salmonella serogroup B isolates. These isolates were tested by a PCR-based assay, and for resistance to five antibiotics: ampicillin, chloramphenicol, streptomycin, sulfonamides, and tetracycline (ACSSuT) for the rapid identification of Salmonella Typhimurium DT104. Upon comparing the antibiotic resistance and PCR results with serotype and phage type data, the sensitivity and specificity for the identification of Salmonella Typhimurium DT104 of both methods were found to be 100%, and 99.6%, respectively. Both methods can be completed within 24 h after obtaining an isolate, while serotyping and phagetyping required more than 5 days to complete.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2004.03.005