Cystatin-like Metastasis-associated Protein mRNA Expression in Human Colorectal Cancer Is Associated with Both Liver Metastasis and Patient Survival

Purpose and Experimental Design: We previously reported that an increased expression of cystatin-like metastasis-associated protein ( CMAP ) mRNA is involved in liver-specific metastasis in a mouse model. We also identified its human homologue and showed that the expression of CMAP in various human...

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Bibliographic Details
Published in:Clinical cancer research 2002-08, Vol.8 (8), p.2591-2594
Main Authors: UTSUNOMIYA, Tohru, HARA, Yoshikazu, KATAOKA, Akemi, MORITA, Masashi, ARAKAWA, Hiroharu, MORI, Masaki, NISHIMURA, Susumu
Format: Article
Language:English
Subjects:
Aged
Biological and medical sciences
Biomarkers, Tumor - biosynthesis
Colorectal Neoplasms - metabolism
Colorectal Neoplasms - mortality
Colorectal Neoplasms - pathology
Cystatins - biosynthesis
Disease-Free Survival
DNA, Complementary - metabolism
Female
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Liver Neoplasms - mortality
Liver Neoplasms - secondary
Male
Medical sciences
Middle Aged
Neoplasm Metastasis
Prognosis
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Time Factors
Tumor Cells, Cultured
Tumors
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Summary:Purpose and Experimental Design: We previously reported that an increased expression of cystatin-like metastasis-associated protein ( CMAP ) mRNA is involved in liver-specific metastasis in a mouse model. We also identified its human homologue and showed that the expression of CMAP in various human cancer cell lines correlated with the description of malignancy in these cell lines. However, there is still no information available on the clinical significance of CMAP expression in human cancer specimens. Thus, we studied the CMAP expression levels using a real-time quantitative reverse transcription-PCR for 79 patients with colorectal cancer, including 17 cases with liver metastasis. Results: The mean expression level of CMAP in tumor tissue specimens was significantly higher than in the corresponding normal tissue specimens ( P < 0.05). A higher expression of CMAP was significantly correlated with liver metastasis ( P < 0.01) as well as with a less differentiated histological type ( P < 0.05) of colorectal cancer. An increased expression of CMAP was also identified as the strongest independent factor for liver metastasis based on a multivariate analysis ( P < 0.001). Furthermore, the prognosis of the patients with a higher expression of CMAP was significantly worse than those with a lower expression (5-year survival rate; 49.7% and 75.0%, respectively, P = 0.038). Conclusions: These findings imply that the expression level of CMAP in human cancer may be a new biomarker for both liver metastasis and the patient’s outcome.
ISSN:1078-0432
1557-3265