Simple method for plant virus detection: effect of antibody immobilization technique

The possibility has been demonstrated for applying a surface plasmon resonance for detecting plant viruses in real samples. An optimal mode for antiviral immunoglobulin immobilization on sensor surfaces is described. Out of three proposed techniques for sensor surface treatment, namely, unmodified g...

Full description

Saved in:
Bibliographic Details
Published in:Journal of virological methods 2002-08, Vol.105 (1), p.141-146
Main Authors: Boltovets, P.M, Boyko, V.R, Kostikov, I.Yu, Dyachenko, N.S, Snopok, B.A, Shirshov, Yu.M
Format: Article
Language:English
Subjects:
Antibodies, Viral - metabolism
Bacteriology
Biological and medical sciences
Chlorophyta - virology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Gold - metabolism
Microbiology
Molecular immunology
Oriented protein immobilization
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Plant Diseases - virology
Staphylococcal Protein A - metabolism
Surface plasmon resonance
Surface Plasmon Resonance - methods
Surface Properties
Techniques
Thiocyanates - metabolism
TMV
Tobacco Mosaic Virus - isolation & purification
Tobacco Mosaic Virus - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The possibility has been demonstrated for applying a surface plasmon resonance for detecting plant viruses in real samples. An optimal mode for antiviral immunoglobulin immobilization on sensor surfaces is described. Out of three proposed techniques for sensor surface treatment, namely, unmodified gold surface, gold surface treated with (a) thiocyanate and (b) thiocyanate and protein A ( Staphylococcus aureus), the latter was chosen as most suited for retention of the formed native immunoglobulin layer.
ISSN:0166-0934
1879-0984
DOI:10.1016/S0166-0934(02)00098-8