The SCF(COI1) ubiquitin-ligase complexes are required for jasmonate response in Arabidopsis

Xie and colleagues previously isolated the Arabidopsis COI1 gene that is required for response to jasmonates (JAs), which regulate root growth, pollen fertility, wound healing, and defense against insects and pathogens. In this study, we demonstrate that COI1 associates physically with AtCUL1, AtRbx...

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Bibliographic Details
Published in:The Plant cell 2002-08, Vol.14 (8), p.1919-1935
Main Authors: Xu, Linghui, Liu, Fuquan, Lechner, Esther, Genschik, Pascal, Crosby, William L, Ma, Hong, Peng, Wen, Huang, Dafang, Xie, Daoxin
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino Acid Substitution
Arabidopsis - drug effects
Arabidopsis - genetics
Arabidopsis - growth & development
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Cell Cycle Proteins - genetics
Cell Cycle Proteins - metabolism
Cullin Proteins
Cyclopentanes - pharmacology
Gene Expression
Ligases - genetics
Ligases - metabolism
Molecular Sequence Data
Mutation
Oxylipins
Peptide Synthases - genetics
Peptide Synthases - metabolism
Plant Growth Regulators - pharmacology
Plants, Genetically Modified
Sequence Homology, Amino Acid
Signal Transduction - drug effects
SKP Cullin F-Box Protein Ligases
Two-Hybrid System Techniques
Ubiquitin-Protein Ligases
Ubiquitins - metabolism
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Summary:Xie and colleagues previously isolated the Arabidopsis COI1 gene that is required for response to jasmonates (JAs), which regulate root growth, pollen fertility, wound healing, and defense against insects and pathogens. In this study, we demonstrate that COI1 associates physically with AtCUL1, AtRbx1, and either of the Arabidopsis Skp1-like proteins ASK1 or ASK2 to assemble ubiquitin-ligase complexes, which we have designated SCF(COI1). COI1(E22A), a single amino acid substitution in the F-box motif of COI1, abolishes the formation of the SCF(COI1) complexes and results in loss of the JA response. AtRbx1 double-stranded RNA-mediated genetic interference reduces AtRbx1 expression and affects JA-inducible gene expression. Furthermore, we show that the AtCUL1 component of SCF(COI1) complexes is modified in planta, where mutations in AXR1 decrease the abundance of the modified AtCUL1 of SCF(COI1) and lead to a reduction in JA response. Finally, we demonstrate that the axr1 and coi1 mutations display a synergistic genetic interaction in the double mutant. These results suggest that the COI1-mediated JA response is dependent on the SCF(COI1) complexes in Arabidopsis and that the AXR1-dependent modification of the AtCUL1 subunit of SCF(COI1) complexes is important for JA signaling.
ISSN:1040-4651
1532-298X
DOI:10.1105/tpc.003368