Invasive front in oral squamous cell carcinoma: Image and flow cytometric analysis with clinicopathologic correlation

Objective. Pathologists have drawn attention to the invasive tumor front (ITF) in the determination of the biologic aggressiveness of oral cancer. We have attempted to discover the prognostic significance of cancer cells with abnormal DNA content at the ITF of oral squamous cell carcinoma. Study Des...

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Published in:Oral surgery, oral medicine, oral pathology, oral radiology and endodontics oral medicine, oral pathology, oral radiology and endodontics, 2002-06, Vol.93 (6), p.682-687
Main Authors: Noguchi, Makoto, Kinjyo, Hisanori, Kohama, Gen-iku, Nakamori, Kenji
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Analysis of Variance
Aneuploidy
Biological and medical sciences
Carcinoma, Squamous Cell - genetics
Carcinoma, Squamous Cell - pathology
Dentistry
DNA, Neoplasm - genetics
Female
Flow Cytometry
Humans
Image Cytometry
Male
Medical sciences
Middle Aged
Mouth Neoplasms - genetics
Mouth Neoplasms - pathology
Neoplasm Invasiveness
Neoplasm Staging
Otorhinolaryngology. Stomatology
Prognosis
Proportional Hazards Models
Statistics, Nonparametric
Survival Analysis
Tumors
Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology
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Summary:Objective. Pathologists have drawn attention to the invasive tumor front (ITF) in the determination of the biologic aggressiveness of oral cancer. We have attempted to discover the prognostic significance of cancer cells with abnormal DNA content at the ITF of oral squamous cell carcinoma. Study Design. A comparative DNA analysis by means of image cytometry and flow cytometry was conducted to confirm the usefulness of image cytometry in detecting cancer cells having abnormal DNA content at the ITF. The prognostic value of cancer cells with abnormal DNA content ws examined by a multivariate analysis for 195 patients with oral squamous cell carcinoma. Results. In the comparative DNA analysis, it was suggested that image cytometry is useful for detecting cancer cells with abnormal DNA content (4c exceeding rate [4cER]), which is associated with poor prognosis of patients with oral squamous cell carcinoma. In the multivariate analysis, 3 independent factors were found to significantly influence cause-specific survival. These are, in decreasing order of influence, (1) abnormal DNA content (4cER), (2) clinical stage, and (3) growth type. Conclusion. The presence of cancer cells with abnormal DNA content of the ITF in conjunction with clinical findings (clinical stage and growth type) can give additional useful information when selecting treatment strategies for oral cancer patients. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002;93:682-7)
ISSN:1079-2104
1528-395X
DOI:10.1067/moe.2002.122341