Influence of C-phycocyanin on hepatocellular parameters related to liver oxidative stress and Kupffer cell functioning

Kupffer cells, liver macrophages involved in immunomodulation, phagocytosis, and biochemical attack, can induce cytotoxicity and inflammation when their activity is exacerbated. The aim of this study was to evaluate the effects of C-phycocyanin on Kupffer cell functioning considering its antioxidant...

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Bibliographic Details
Published in:Inflammation research 2002-07, Vol.51 (7), p.351-356
Main Authors: Remirez, D, Fernández, V, Tapia, G, González, R, Videla, L A
Format: Article
Language:English
Subjects:
Animals
Carbon - metabolism
Enzyme-Linked Immunosorbent Assay
Hepatocytes - drug effects
Hepatocytes - metabolism
In Vitro Techniques
Kupffer Cells - drug effects
Kupffer Cells - metabolism
L-Lactate Dehydrogenase - metabolism
Liver - drug effects
Liver - metabolism
Mice
Nitric Oxide Synthase - metabolism
Nitrites - blood
Oxidative Stress - drug effects
Oxygen Consumption - drug effects
Phycocyanin - pharmacology
Rats
Respiratory Burst - drug effects
Triiodothyronine - pharmacology
Tumor Necrosis Factor-alpha - metabolism
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Summary:Kupffer cells, liver macrophages involved in immunomodulation, phagocytosis, and biochemical attack, can induce cytotoxicity and inflammation when their activity is exacerbated. The aim of this study was to evaluate the effects of C-phycocyanin on Kupffer cell functioning considering its antioxidant and anti-inflammatory properties. Actions of C-phycocyanin on colloidal carbon phagocytosis, carbon-induced respiratory burst activity, and sinusoidal lactate dehydrogenase (LDH) release were studied in isolated perfused mouse liver. The influence of C-phycocyanin on tumor necrosis factor-a (TNF-alpha) and nitrite levels in serum and liver nitric oxide synthase (NOS) activity was assessed in rats subjected to thyroid hormone (T3) administration, a condition known to underlie hepatic oxidative stress comprising an increased Kupffer cell activity. C-phycocyanin elicited a concentration-dependent inhibition of carbon phagocytosis and carbon-induced O2 uptake (IC50 = 0.2 mg/ml) by perfused livers, with a 52% diminution in the carbon-induced sinusoidal release of LDH being found at a concentration of 0.25 mg/ml. Thyroid calorigenesis induced an 82-fold increase in serum TNF-alpha levels, an effect that was suppressed by pretreatment with C-phycocyanin, the antioxidant alpha-tocopherol, and by the Kupffer cell inactivator gadolinium chloride. C-phycocyanin also suppressed the T3-induced increases in serum nitrite levels (234%) and in the activity of hepatic NOS (75%). C-phycocyanin significantly decreases Kupffer cell phagocytosis and the associated respiratory burst activity, effects that may contribute to the abolition of oxidative stress-induced TNF-alpha response and NO production by hyperthyroid state.
ISSN:1023-3830
1420-908X
DOI:10.1007/PL00000314