Overexpression of the erbB-2 Proto-oncogene in Canine Osteosarcoma Cell Lines and Tumors

The status of the erbB-2 (human epidermal growth factor receptor 2/neu) proto-oncogene in canine osteosarcoma (OSA) has not been reported previously. In this study we used real-time reverse transcriptase polymerase chain reaction to evaluate erbB-2 expression in seven canine OSA cell lines and 10 ca...

Full description

Saved in:
Bibliographic Details
Published in:Veterinary pathology 2004-05, Vol.41 (3), p.291-296
Main Authors: Flint, A. F., U'Ren, L., Legare, M. E., Withrow, S. J., Dernell, W., Hanneman, W. H.
Format: Article
Language:English
Subjects:
Animals
Cell Line, Tumor
Disease Models, Animal
DNA Primers
Dogs
Gene Expression Regulation, Neoplastic
Genes, erbB-2
Histological Techniques
Humans
Lymph Nodes - pathology
Osteosarcoma - metabolism
Osteosarcoma - pathology
Receptor, ErbB-2 - metabolism
Reverse Transcriptase Polymerase Chain Reaction
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The status of the erbB-2 (human epidermal growth factor receptor 2/neu) proto-oncogene in canine osteosarcoma (OSA) has not been reported previously. In this study we used real-time reverse transcriptase polymerase chain reaction to evaluate erbB-2 expression in seven canine OSA cell lines and 10 canine OSA tissue samples. We determined erbB-2 to be significantly overexpressed in 86% (six of seven) of the cell lines and 40% (4 of 10) of the OSA tissues samples. Given the importance of erbB-2 in human breast cancer, the finding of erbB-2 overexpression in canine OSA may be important in further understanding the pathogenesis and possible therapies of OSA.
ISSN:0300-9858
1544-2217
DOI:10.1354/vp.41-3-291