An in vitro model for purging of tumour cells from ovarian tissue

BACKGROUND: Cryopreservation and autografting of ovarian tissue may preserve fertility after cancer treatment, but could be hampered by tumour cell contamination. Epithelial tumour cell lysis can be obtained with cytotoxic T cell retargeting through the bispecific antibody BIS‐1, with combined affin...

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Bibliographic Details
Published in:Human reproduction (Oxford) 2004-05, Vol.19 (5), p.1069-1075
Main Authors: Schröder, C.P., Timmer‐Bosscha, H., Wijchman, J.G., de Leij, L.F.M.H., Hollema, H., Heineman, M.J., de Vries, E.G.E.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Antibodies, Bispecific
Biological and medical sciences
Breast Neoplasms - immunology
Breast Neoplasms - prevention & control
cancer/fertility/ovary/tumour cell lysis
Cell Death
Cell Line, Tumor
Cell Separation - methods
Cryopreservation
Embryology: invertebrates and vertebrates. Teratology
Epithelial Cells - pathology
Female
Fundamental and applied biological sciences. Psychology
Humans
In Vitro Techniques
Infertility, Female - therapy
Ovarian Follicle - cytology
Ovarian Follicle - pathology
Ovarian Follicle - transplantation
Precursor Cell Lymphoblastic Leukemia-Lymphoma - immunology
Precursor Cell Lymphoblastic Leukemia-Lymphoma - prevention & control
T-Lymphocytes, Cytotoxic - cytology
T-Lymphocytes, Cytotoxic - immunology
Transplantation, Autologous
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Summary:BACKGROUND: Cryopreservation and autografting of ovarian tissue may preserve fertility after cancer treatment, but could be hampered by tumour cell contamination. Epithelial tumour cell lysis can be obtained with cytotoxic T cell retargeting through the bispecific antibody BIS‐1, with combined affinity for the T‐cell receptor and epithelial glycoprotein‐2 (EGP‐2). Our aim was to study the concept of tumour cell purging in the setting of a suspension of ovarian tissue. METHODS: Human ovarian tissue was brought into suspension by mechanical and enzymatic treatment. Cells of the MCF‐7 breast cancer cell line and activated human lymphocytes were co‐ incubated for 4 h with or without BIS‐1 and with or without ovarian suspension. After incubation, MCF‐7 cell death and cell growth were evaluated by fluorescent cell detection and MTT assay, respectively. Ovarian tissue morphology was evaluated immunohistochemically. RESULTS: MCF‐7 cell death and cell growth inhibition increased with increasing ratios of lymphocytes to MCF‐7 cells. BIS‐1 addition gave further augmentation, with a maximum depletion of growing MCF‐7 cells of 89% (SD 11%) versus without BIS‐1, 23% (SD 15%; P 
ISSN:0268-1161
1460-2350
1460-2350
DOI:10.1093/humrep/deh244