Ligand‐activated natural killer T lymphocytes promptly produce IL‐3 and GM‐CSF in vivo: relevance to peripheral myeloid recruitment

Natural killer (NK) T cells are prominent for their prompt IL‐4 and IFN‐γ production upon TCR ligation that enables them to influence acquired immune responses. In the present study we provide evidence that the regulatory functions of this particular T cell subset extend to the myeloid compartment o...

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Bibliographic Details
Published in:European journal of immunology 2002-07, Vol.32 (7), p.1897-1904
Main Authors: Leite‐de‐Moraes, Maria C., Lisbonne, Mariette, Arnould, Anne, Machavoine, François, Herbelin, André, Dy, Michel, Schneider, Elke
Format: Article
Language:English
Subjects:
Animals
CD1d
Colony‐forming unit cell
Galactosylceramides - administration & dosage
Galactosylceramides - immunology
Granulocyte-Macrophage Colony-Stimulating Factor - biosynthesis
Granulocyte-Macrophage Colony-Stimulating Factor - genetics
Granulocytes - cytology
Granulocytes - immunology
Hematopoiesis, Extramedullary
Injections
Interferon-gamma - genetics
Interleukin-3 - biosynthesis
Interleukin-3 - genetics
Interleukin-4 - genetics
Killer Cells, Natural - drug effects
Killer Cells, Natural - immunology
Ligands
Mice
Mice, Inbred C57BL
Mice, Knockout
Myeloid Cells - cytology
Myeloid Cells - immunology
Neutrophil
NKT lymphocyte
T-Lymphocytes - drug effects
T-Lymphocytes - immunology
α‐Galactosylceramide
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Summary:Natural killer (NK) T cells are prominent for their prompt IL‐4 and IFN‐γ production upon TCR ligation that enables them to influence acquired immune responses. In the present study we provide evidence that the regulatory functions of this particular T cell subset extend to the myeloid compartment of bone marrow and spleen through its production of hematopoietic growth factors. Bone marrow and spleen NKT cells responded to a single injection of their specific ligand α‐galactosylceramide (α‐GalCer) by producing both IL‐3 and granulocyte‐macrophage colony stimulating factor (GM‐CSF), whose colony‐stimulating activity became detectable in the serum as early as 1 h post treatment. These cytokines were not produced in mice lacking NKT cells (CD1d–/–), whose exclusive involvement in this biological activity was further confirmed by intracellular immuno‐staining. Growth factor production was accompanied by significant changes in the myeloid compartment of treated mice, namely mobilization of myeloid progenitors (colony‐forming unit cells, CFU‐C) and neutrophils from the bone marrow to the periphery. Taken together, our data support the notion that activated NKT cells influence innate immune responses by recruiting myeloid progenitors and granulocytes to the periphery through their production of hematopoietic growth factors.
ISSN:0014-2980
1521-4141
DOI:10.1002/1521-4141(200207)32:7<1897::AID-IMMU1897>3.0.CO;2-Y