Erythropoietin and G-CSF receptors in human tumor cells: expression and aspects regarding functionality

Recombinant human erythropoietin (Epo) and granulocyte-colony-stimulating factor (G-CSF) are used to stimulate hematopoiesis in patients with malignant diseases. These cytokines transduce their biological signal via the Epo receptor (EpoR) and G-CSF receptor (G-CSF-R) into the cell. We therefore inv...

Full description

Saved in:
Bibliographic Details
Published in:Tumori 2002-03, Vol.88 (2), p.150-159
Main Authors: Westphal, Gabriela, Niederberger, Ellen, Blum, Christoph, Wollman, Yoram, Knoch, Tobias A, Rebel, Wolfgang, Debus, Jürgen, Friedrich, Eckhard
Format: Article
Language:English
Subjects:
Blotting, Northern
Blotting, Western
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Neoplasms - physiopathology
Receptors, Erythropoietin - biosynthesis
Receptors, Erythropoietin - physiology
Receptors, Granulocyte Colony-Stimulating Factor - biosynthesis
Receptors, Granulocyte Colony-Stimulating Factor - physiology
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Recombinant human erythropoietin (Epo) and granulocyte-colony-stimulating factor (G-CSF) are used to stimulate hematopoiesis in patients with malignant diseases. These cytokines transduce their biological signal via the Epo receptor (EpoR) and G-CSF receptor (G-CSF-R) into the cell. We therefore investigated in human tumor cell lines the expression of these receptors in tumor cells as well as their response to Epo and G-CSF. The expression of EpoR and G-CSF-R mRNA was analyzed with reverse transcription-polymerase chain reaction (RT-PCR). EpoR protein expression was further monitored with Western blot and immunocytochemistry analysis. The cellular response to various concentrations of Epo was evaluated using 3[H]-thymidine uptake, Northern blot of c-fos expression and tyrosine kinase activity assay. The proliferation after G-CSF incubation was analyzed with the MTS assay. In this study EpoR mRNA and protein were detected in various human tumor cell lines. Treatment with Epo did not influence the proliferation rate of examined EpoR-positive tumor cell lines. Epo did not stimulate the tyrosine kinase activity nor did it affect the c-fos mRNA in these cell lines. G-CSF-R mRNA was only detected in two myeloid cell lines. Treatment with G-CSF did not increase the proliferation of these cells. These results demonstrate that Epo and G-CSF did not modulate the growth rate of examined receptor-positive tumor cell lines; the presence of the Epo receptor seems not essential for cell growth of these tumor cells in cell culture.
ISSN:0300-8916
2038-2529
DOI:10.1177/030089160208800214