Identification of major allergens of Malassezia pachydermatis in dogs with atopic dermatitis and Malassezia overgrowth

We have previously shown that both atopic and normal dogs generate an IgG response to antigens of Malassezia pachydermatis. The aim of this study was to compare IgE responses to separated proteins of M. pachydermatis in 28 atopic dogs with Malassezia dermatitis and 22 clinically normal dogs using We...

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Bibliographic Details
Published in:Veterinary dermatology 2002-06, Vol.13 (3), p.141-150
Main Authors: Chen, Tai-An, Halliwell, Richard E. W., Pemberton, Alan D., Hill, Peter B.
Format: Article
Language:English
Subjects:
Allergens - blood
Animals
atopic dermatitis
Blotting, Western - veterinary
Dermatitis, Atopic - complications
Dermatitis, Atopic - immunology
Dermatitis, Atopic - veterinary
Dermatomycoses - complications
Dermatomycoses - immunology
Dermatomycoses - veterinary
Dog Diseases - immunology
Dog Diseases - microbiology
dogs
Dogs - immunology
Dogs - microbiology
Electrophoresis, Polyacrylamide Gel - veterinary
IgE
Immunoglobulin E - blood
Malassezia
Malassezia - immunology
Malassezia - isolation & purification
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Summary:We have previously shown that both atopic and normal dogs generate an IgG response to antigens of Malassezia pachydermatis. The aim of this study was to compare IgE responses to separated proteins of M. pachydermatis in 28 atopic dogs with Malassezia dermatitis and 22 clinically normal dogs using Western immunoblotting. Six different detection systems were evaluated in order to assess sensitivity and eliminate nonspecific binding and cross‐reactivity. The protocol yielding the best results utilized a monoclonal mouse antidog IgE, an alkaline phosphatase conjugated goat antimouse IgG which had been passed through a canine IgG column 3 times, a chemiluminescent substrate and a digital imaging system. Proteins of 45, 52, 56 and 63 kDa were recognized by more than 50% of the atopic dog sera and thus represented major allergens. Only a minority of normal dogs showed faint IgE binding to these proteins. The results indicate that the majority of atopic dogs with Malassezia dermatitis have a greater IgE response than normal dogs, suggesting an IgE‐mediated immune response may be clinically important in the pathogenesis of the disease.
ISSN:0959-4493
1365-3164
DOI:10.1046/j.1365-3164.2002.00291.x