ANGPTL3 is increased in both insulin-deficient and -resistant diabetic states

Angiopoietin-like protein 3 (ANGPTL3) is secreted by the liver and was recently postulated to be an important hormone regulating serum triglyceride levels. In the present study, in order to clarify the regulation of ANGPTL3 gene expression in diabetic states, we examined mRNA and protein levels of A...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2004-05, Vol.317 (4), p.1075-1079
Main Authors: Inukai, Kouichi, Nakashima, Youhei, Watanabe, Masaki, Kurihara, Susumu, Awata, Takuya, Katagiri, Hideki, Oka, Yoshitomo, Katayama, Shigehiro
Format: Article
Language:English
Subjects:
Angiopoietin-like Proteins
Angiopoietins
ANGPTL3
Animals
Blood Glucose - metabolism
Blotting, Northern
Cholesterol - blood
COS Cells
db/db mice
Diabetes Mellitus, Experimental - metabolism
Fatty Acids, Nonesterified - blood
Gene Expression Regulation - drug effects
Humans
Insulin - deficiency
Insulin - pharmacology
Insulin Resistance - physiology
Intercellular Signaling Peptides and Proteins - biosynthesis
Intercellular Signaling Peptides and Proteins - genetics
Liver - metabolism
Male
Mice
Mice, Inbred C57BL
Mice, Mutant Strains
STZ mice
Transfection
Triglycerides - blood
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Summary:Angiopoietin-like protein 3 (ANGPTL3) is secreted by the liver and was recently postulated to be an important hormone regulating serum triglyceride levels. In the present study, in order to clarify the regulation of ANGPTL3 gene expression in diabetic states, we examined mRNA and protein levels of ANGPTL3 in the livers of diabetic animals. The level of ANGPTL3 mRNA was increased approximately 2.2-fold in the livers of streptozotocin (STZ) diabetic mice, and this effect was reversed by administration of insulin. Furthermore, the level of ANGPTL3 mRNA was increased more than 3.0-fold in type 2 diabetic obese mice, db/db mice, as compared with age matched lean littermates. The hepatic level of ANGPTL3 protein was also increased in these diabetic mice to an extent similar to that of ANGPTL3 mRNA. Thus, the expression of ANGPTL3 was enhanced in both insulin-deficient and -resistant diabetic states. These results strongly suggest ANGPTL3 to play an important role in hyperlipidemia in diabetes.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2004.03.151