Non-conventional yeasts as producers of polyhydroxyalkanoates--genetic engineering of Arxula adeninivorans

The non-conventional yeast Arxula adeninivorans was equipped with the genes phbA, phbB and phbC of the polyhydroxyalkanoate (PHA) biosynthetic pathway of Ralstonia eutropha, which encode β-ketothiolase, NADPH-linked acetoacetyl-CoA reductase and PHA synthase, respectively. Arxula strains transformed...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2004-04, Vol.64 (3), p.376-381
Main Authors: Terentiev, Y, Breuer, U, Babel, W, Kunze, G
Format: Article
Language:English
Subjects:
Acetyl-CoA C-Acyltransferase - genetics
Acetyl-CoA C-Acyltransferase - physiology
Acyltransferases - genetics
Acyltransferases - physiology
Alcohol Oxidoreductases - genetics
Alcohol Oxidoreductases - physiology
Arxula adeninivorans
Bacteria
Biological and medical sciences
biopolymers
Biotechnology
Cloning, Molecular
Culture Media
Cupriavidus necator - enzymology
Cupriavidus necator - genetics
Ethanol - metabolism
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genetic Engineering
genetic transformation
Genetic Vectors
genetically modified organisms
Hydroxybutyrates - metabolism
Kinetics
Microbiology
Plasmids - genetics
Polyesters - metabolism
polyhydroxyalkanoates
Ralstonia eutropha
Saccharomycetales - genetics
Saccharomycetales - metabolism
Transformation, Genetic
Valerates - metabolism
Yeasts - genetics
Yeasts - metabolism
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Summary:The non-conventional yeast Arxula adeninivorans was equipped with the genes phbA, phbB and phbC of the polyhydroxyalkanoate (PHA) biosynthetic pathway of Ralstonia eutropha, which encode β-ketothiolase, NADPH-linked acetoacetyl-CoA reductase and PHA synthase, respectively. Arxula strains transformed solely with the PHA synthase gene (phbC) were able to produce PHA. However, the maximum content of the polymer detected in these strains was just 0.003% poly-3-hydroxybutyrate (PHB) and 0.112% poly-3-hydroxyvalerate (PHV). The expression of all three genes (phbA, phbB, phbC) resulted in small increases in the PHA content of the transgenic Arxula cells. However, under controlled cultivation conditions with minimal medium and ethanol as the carbon source, the recombinant yeast was able to accumulate up to 2.2% PHV and 0.019% PHB. Possible reasons for these differences are discussed.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-003-1498-x