Effector mechanisms in murine allograft rejection: comparison of skin and heart grafts in fully allogeneic and minor histocompatibility antigen‐mismatched strain combinations

Cytotoxic T lymphocytes (CTLs) and macrophage‐mediated delayed‐type hypersensitivity (DTH) responses may both mediate allograft rejection. Furthermore, although allograft rejection is classically considered a type [22, 23, 38, 50, 52] 1 cellular immune response, type‐2 cytokines can support rejectio...

Full description

Saved in:
Bibliographic Details
Published in:Transplant international 2002-06, Vol.15 (6), p.302-309
Main Authors: Youssef, Abdel‐Rahman, Otley, Carolyn, Mathieson, Peter W., Smith, Richard M.
Format: Article
Language:English
Subjects:
Allograft rejection
Animals
Biological and medical sciences
Cytokine
Cytokines - genetics
Cytotoxic T cell
Delayed‐type hypersensitivity
Effector mechanism
Gene Expression Profiling
General aspects
Graft Rejection - etiology
Graft Survival
Heart Transplantation - immunology
Histocompatibility Testing
Hypersensitivity, Delayed - etiology
Immunopathology
Male
Medical sciences
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Minor Histocompatibility Antigens - immunology
Myocardium - pathology
Reverse Transcriptase Polymerase Chain Reaction
Skin - pathology
Skin Transplantation - immunology
T-Lymphocytes, Cytotoxic - immunology
Transplantation, Homologous
Transplantation, Isogeneic
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cytotoxic T lymphocytes (CTLs) and macrophage‐mediated delayed‐type hypersensitivity (DTH) responses may both mediate allograft rejection. Furthermore, although allograft rejection is classically considered a type [22, 23, 38, 50, 52] 1 cellular immune response, type‐2 cytokines can support rejection. This study examines whether the immunogenicity of the transplanted tissue, as determined by type of tissue (skin versus heart) and degree of antigenic mismatch, influences recruitment of these effector mechanisms. Graft survival, histological appearance and intragraft gene expression (IL‐2, IFN‐γ, IL‐12 p40, IL‐4, IL‐10, perforin, Fas ligand (Fas L), iNOS and TNF‐α) were compared for fully allogeneic, minor histocompatibility (mHC) antigen‐mismatched and syngeneic skin and heart grafts. We found mRNA characteristic of CTLs and DTH responses in fully allogeneic and mHC antigen‐mismatched skin and heart grafts. Concomitant type‐1 and type‐2 cytokine gene transcription was seen. These findings demonstrate that the tissue grafted and degree of antigenic disparity between donor and recipient do not restrict the repertoire of cellular immune responses involved in graft rejection. This finding has implications in the design of new immuno‐suppressive strategies for clinical transplantation.
ISSN:0934-0874
1432-2277
DOI:10.1111/j.1432-2277.2002.tb00169.x