Glutamine metabolism in ovine splanchnic tissues: effects of infusion of ammonium bicarbonate or amino acids into the abomasum

This study investigates the effects of increased NH3 or amino acid supply on glutamine utilisation and production by the splanchnic tissues of fed sheep. Six sheep, prepared with vascular catheters in the aorta, mesenteric, portal and hepatic veins, were fed grass pellets to 1·1×energy maintenance r...

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Bibliographic Details
Published in:British journal of nutrition 2002-04, Vol.87 (4), p.357-366
Main Authors: Nieto, R., Obitsu, T., Fernández-Quintela, A., Bremner, D., Milne, E., Calder, A. G., Lobley, G. E.
Format: Article
Language:English
Subjects:
15N kinetics
Abomasum
Amino acids
Amino Acids - administration & dosage
Ammonia
Animals
Bicarbonates - administration & dosage
Biological and medical sciences
Catheters
Coronary vessels
Fundamental and applied biological sciences. Psychology
Glutamine
Glutamine - metabolism
Liver
Male
Metabolism
Portal-drained viscera
Sheep
Sheep - metabolism
Splanchnic Circulation
Stomach
Ureagenesis
Veins & arteries
Vertebrates: digestive system
Viscera - blood supply
Viscera - metabolism
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Summary:This study investigates the effects of increased NH3 or amino acid supply on glutamine utilisation and production by the splanchnic tissues of fed sheep. Six sheep, prepared with vascular catheters in the aorta, mesenteric, portal and hepatic veins, were fed grass pellets to 1·1×energy maintenance requirements. Each treatment involved a 4 d abomasal infusion, of either ammonium bicarbonate (AMM; 23·4 μmol/kg0·75 per min), water (CONT), or a mixture of amino acids that excluded glutamine and glutamate (AA; 46·8 μmol amino acid-N/kg0·75 per min). The treatments simulated nutritional extremes in terms of the balance of absorbed N. Kinetics across the whole gut and the liver were monitored during an intra-jugular infusion of [5-15N]glutamine. Blood flow across the whole gut or liver were unaffected by treatment. Both AMM and AA infusions doubled the hepatic release of urea-N compared with CONT (P
ISSN:0007-1145
1475-2662
DOI:10.1079/BJN2002525