Investigation of the Aedes (Stegomyia) flavopictus Complex (Diptera: Culicidae) in Japan by Sequence Analysis of the Internal Transcribed Spacers of Ribosomal DNA

Aedes (Stegomyia) flavopictus Yamada is widely distributed in Japan and Korea. The species comprises three subspecies based on current morphological taxonomy: Aedes f. flavopictus in the Palearctic region of Japan, Ae. f. downsi Bohart & Ingram from Amami and Okinawa Islands, and Ae. f. miyarai...

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Bibliographic Details
Published in:Journal of medical entomology 2002-05, Vol.39 (3), p.461-468
Main Authors: Toma, Takako, Miyagi, Ichiro, Crabtree, Mary B., Miller, Barry R.
Format: Article
Language:English
Subjects:
Aedes - classification
Aedes - genetics
Aedes flavopictus
Aedes flavopictus downsi
Aedes flavopictus miyarai
Animals
Base Sequence
Biological and medical sciences
DNA, Complementary
DNA, Ribosomal Spacer - analysis
Female
Fundamental and applied biological sciences. Psychology
Insecta
Invertebrates
Japan
Male
Medically important nuisances and vectors, pests of stored products and materials: population survey and control
Molecular Sequence Data
phylogenetic tree
Phylogeny
ribosomal DNA
Sequence Analysis, DNA
Systematics. Geographical distribution
Vectors. Intermediate hosts
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Summary:Aedes (Stegomyia) flavopictus Yamada is widely distributed in Japan and Korea. The species comprises three subspecies based on current morphological taxonomy: Aedes f. flavopictus in the Palearctic region of Japan, Ae. f. downsi Bohart & Ingram from Amami and Okinawa Islands, and Ae. f. miyarai Tanaka, Mizusawa & Ingram from Ishigaki and Iriomote Islands of the Ryukyu Archipelago. These subspecies designations are based on observations of a combination of several morphological characters, none of which, by itself is diagnostic for discriminating among the three subspecies. To further study the relationships in this group, we examined the nucleic acid sequence divergence in the internal transcribed spacer regions (ITS1 and ITS2) of the ribosomal DNA gene array of Ae. flavopictus individuals collected at five sites from three geographic regions in Japan. Analysis of sequence data by distance and maximum parsimony methods produced phylogenetic trees that showed separation of the specimens into three major clades, corresponding to both subspecies and geographic region. These results were consistent with and support the current classification and geographic distribution of the three subspecies.
ISSN:0022-2585
1938-2928
DOI:10.1603/0022-2585-39.3.461