Microarray-Based in vitro Evaluation of DNA Oligomer Libraries Designed in silico

We report on the microarray‐based in vitro evaluation of two libraries of DNA oligonucleotide sequences, designed in silico for applications in supramolecular self‐assembly, such as DNA computing and DNA‐based nanosciences. In this first study which is devoted to the comparison of sequence motif pro...

Full description

Saved in:
Bibliographic Details
Published in:Chemphyschem 2004-03, Vol.5 (3), p.367-372
Main Authors: Feldkamp, Udo, Wacker, Ron, Schroeder, Hendrik, Banzhaf, Wolfgang, Niemeyer, Christof M.
Format: Article
Language:English
Subjects:
Base Sequence
bioinformatics
Biological and medical sciences
Computational Biology
Databases as Topic
Diverse techniques
Fundamental and applied biological sciences. Psychology
Gene Library
Image Processing, Computer-Assisted
microarrays
Microscopy, Fluorescence
Models, Biological
Molecular and cellular biology
nanostructures
Nanotechnology - methods
Nucleic Acid Hybridization
nucleic acids
Oligodeoxyribonucleotides - analysis
Oligodeoxyribonucleotides - chemistry
Oligonucleotide Array Sequence Analysis - methods
self-assembly
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We report on the microarray‐based in vitro evaluation of two libraries of DNA oligonucleotide sequences, designed in silico for applications in supramolecular self‐assembly, such as DNA computing and DNA‐based nanosciences. In this first study which is devoted to the comparison of sequence motif properties theoretically predicted with their performance in real‐life, the DNA‐directed immobilization (DDI) of proteins was used as an example of DNA‐based self‐assembly. Since DDI technologies, DNA computing, and DNA nanoconstruction essentially depend on similar prerequisites, in particular, large and uniform hybridization efficiencies combined with low nonspecific cross‐reactivity between individual sequences, we anticipate that the microarray approach demonstrated here will enable rapid evaluation of other DNA sequence libraries. From computers to real life: A library of DNA oligomer sequences designed for efficient and specific hybridization with a software tool in silico was experimentally evaluated in vitro by employing DNA microarray technology (see graphic). This approach can be used in a wide variety of applications, including DNA‐based nanotechnology and DNA computing.
ISSN:1439-4235
1439-7641
DOI:10.1002/cphc.200300978