Delivery of a Genetically Marked Alcaligenes sp. to the Glassy-Winged Sharpshooter for Use in a Paratransgenic Control Strategy

An artificial feeding system was designed for the glassy-winged sharpshooter (GWSS), Homalodisca coagulata Say (Hemiptera: Cicadellidae). The system, unlike previous systems, provided enough nutrients to GWSS to survive for 48 h. A system like this is a prerequisite to examining the potential use of...

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Bibliographic Details
Published in:Current microbiology 2004-05, Vol.48 (5), p.327-331
Main Authors: Bextine, B, Lauzon, C, Potter, S, Lampe, D, Miller, T.A
Format: Article
Language:English
Subjects:
Achromobacter xylosoxidans subsp. denitrificans
Alcaligenes - genetics
Alcaligenes - growth & development
Alcaligenes - metabolism
Alcaligenes xylosoxidans
Animals
Antibiosis
Bacteria
Crop diseases
Digestive System - microbiology
DNA Transposable Elements
endosymbionts
Genes, Reporter
genetically engineered microorganisms
Hemiptera - microbiology
Homalodisca vitripennis
insect vectors
Insect Vectors - microbiology
Luminescent Proteins - genetics
Luminescent Proteins - metabolism
Pierce's disease
Plant Diseases - microbiology
plant pathogenic bacteria
Xylella - physiology
Xylella fastidiosa
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Summary:An artificial feeding system was designed for the glassy-winged sharpshooter (GWSS), Homalodisca coagulata Say (Hemiptera: Cicadellidae). The system, unlike previous systems, provided enough nutrients to GWSS to survive for 48 h. A system like this is a prerequisite to examining the potential use of paratransgenesis to interrupt transmission of Xylella fastidiosa, the bacterial pathogen causing Pierce's disease of grape, by insect vectors. We developed a system for short-term feeding of GWSS that allows for the introduction of bacteria in liquid medium, and we have demonstrated the ability of Alcaligenes xylosoxidans denitrificans, expressing a red fluorescent protein (dsRed), to colonize the cibarial region of the GWSS foregut for up to 5 weeks post-exposure. Alcaligenes xylosoxidans denitrificans thus occupies the same region in the foregut as the pathogen, Xylella fastidiosa.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-003-4178-2