Expression of glycogen synthase kinase-3 isoforms in mouse tissues and their transcription in the brain

Glycogen synthase kinase-3α and -3β (GSK-3α and -3β) are multi-substrate, serine/threonine-specific kinases that can phosphorylate microtubule-associated protein tau and other neuronal proteins. In this study, the expression level and mRNA distribution of two GSK-3 isoforms, GSK-3α and -3β in mice w...

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Bibliographic Details
Published in:Journal of chemical neuroanatomy 2002-05, Vol.23 (4), p.291-297
Main Authors: Yao, Hong-Bing, Shaw, Pang-Chui, Wong, Chun-Cheung, Wan, David Chi-Cheong
Format: Article
Language:English
Subjects:
Alzheimer's disease
Amino Acid Sequence
Animals
Animals, Newborn
Brain - enzymology
Brain - physiology
Calcium-Calmodulin-Dependent Protein Kinases - biosynthesis
Gene Expression Regulation, Developmental - physiology
Gene Expression Regulation, Enzymologic - physiology
Glycogen Synthase Kinase 3
Glycogen Synthase Kinases
In situ hybridization
Isoenzymes - biosynthesis
Male
Mice
Mice, Inbred ICR
Molecular Sequence Data
Mouse brain
RNA, Messenger - biosynthesis
Transcription
Transcription, Genetic - physiology
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Summary:Glycogen synthase kinase-3α and -3β (GSK-3α and -3β) are multi-substrate, serine/threonine-specific kinases that can phosphorylate microtubule-associated protein tau and other neuronal proteins. In this study, the expression level and mRNA distribution of two GSK-3 isoforms, GSK-3α and -3β in mice were investigated. Northern blot analyses indicated that GSK-3α mRNA is encoded by a 2.5-kb transcript in adult tissues, whereas a 4.1-kb transcript was found in neonatal tissues. The GSK-3β mRNA is encoded by a 1.6-kb transcript in the testis and a 7.6-kb transcript in the brain, and in many other adult tissues, but not neonatal tissues. Western blot analyses demonstrated that GSK-3β protein was mainly expressed in the brain and heart, whereas GSK-3α was highly expressed in the brain, heart, and testis. A non-radioactive in situ hybridization study using specific digoxigenin-labeled RNA probes showed that GSK-3α and -3β mRNAs were found in many brain regions, and were especially abundant in the hippocampus, cerebral cortex, and the Purkinje cells of the cerebellum. This implies the importance of GSK-3α and -3β for brain function. The differential expression of GSK-3α and -3β mRNAs as well as proteins in other tissues indicate that they play different roles in cellular functions and the developmental process.
ISSN:0891-0618
1873-6300
DOI:10.1016/S0891-0618(02)00014-5