Three‐dimensional architecture and distribution of collagen components in the goat hypophysis

The three‐dimensional architecture of collagen fibrils in the connective tissue framework and the distribution of collagen types in the goat hypophysis were studied by the cell maceration method in combination with scanning electron microscopy (SEM) and immunohistochemistry. The pars distalis of the...

Full description

Saved in:
Bibliographic Details
Published in:The Anatomical record 2004-04, Vol.277A (2), p.275-286
Main Authors: Nishimura, Shotaro, Tabata, Shoji, Nakamura, Yoshi‐Nori, Okano, Kaoru, Iwamoto, Hisao
Format: Article
Language:English
Subjects:
Animals
collagen
Collagen - chemistry
Collagen - classification
Collagen - ultrastructure
Female
goat
Goats - anatomy & histology
hypophysis
Imaging, Three-Dimensional
immunohistochemistry
Male
Pituitary Gland, Anterior - anatomy & histology
Pituitary Gland, Anterior - chemistry
Pituitary Gland, Anterior - ultrastructure
Pituitary Gland, Posterior - anatomy & histology
Pituitary Gland, Posterior - chemistry
Pituitary Gland, Posterior - ultrastructure
scanning electron microscopy
Tissue Distribution
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The three‐dimensional architecture of collagen fibrils in the connective tissue framework and the distribution of collagen types in the goat hypophysis were studied by the cell maceration method in combination with scanning electron microscopy (SEM) and immunohistochemistry. The pars distalis of the adenohypophysis consisted of many cell clusters. SEM revealed that the wall of cell clusters appeared as various‐sized flat bundles of collagen fibrils woven in a basket‐like configuration. In the pars tuberalis, the aggregates of collagen fibrils were denser and bundles thicker compared to the pars distalis. The density of collagen fibrils changed from the pars tuberalis to pars distalis without a distinct border. The collagen framework in the pars intermedia was mainly divided into three parts, the dorsal region with large hollows, the middle region, and the ventral sheet facing the cavum hypophysis. In the lobus nervosus of the neurohypophysis, the collagen network exhibited a sponge‐like appearance at low magnification. Collagen fibrils of various sizes consisted of loose wavy bundles distributed around the cavities. Immunohistochemistry revealed types I, III, IV, V, and VI collagen throughout the hypophysis. It is concluded that to maintain structural and functional integration, the components of collagen are in different configurations throughout the regions of the goat hypophysis. Anat Rec Part A 277A:275–286, 2004. © 2004 Wiley‐Liss, Inc.
ISSN:1552-4884
0003-276X
1552-4892
1097-0185
DOI:10.1002/ar.a.20014