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Quality control of benserazide-levodopa and carbidopa-levodopa tablets by capillary zone electrophoresis
In modern practice, the treatment of Parkinson's disease and syndrome is carried out using pharmaceutical formulations containing a combination of levodopa and a decarboxylation inhibitor (carbidopa or benserazide). Two pharmaceutical formulations were quantified by capillary zone electrophores...
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Published in: | Electrophoresis 2000-07, Vol.21 (12), p.2432-2437 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites |
Online Access: | Get full text |
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Summary: | In modern practice, the treatment of Parkinson's disease and syndrome is carried out using pharmaceutical formulations containing a combination of levodopa and a decarboxylation inhibitor (carbidopa or benserazide). Two pharmaceutical formulations were quantified by capillary zone electrophoresis using two procedures which differed only in the kind of background electrolyte used. One procedure used a 25 mM phosphate buffer, pH 2.5, while the second one used a 25 mM borate buffer, pH 8.5. The electrophoretic analysis was carried out using an uncoated fused‐ silica capillary, a separation voltage of 20 kV with currents typically less than 60 μA, and spectrophotometric detection at 205 nm. Calibration curves were performed for levodopa (concentration range 1—100 μg/mL), for carbidopa and benserazide (1—50 μg/mL), and the plots of the peak area versus concentration were found to be linear with a correlation coefficient better than 0.9990. Satisfactory results were obtained when commercial tablets were analyzed in terms of accuracy (98—102%), repeatability (0.6—2.0%), and intermediate precision (1.1—2.6%). |
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ISSN: | 0173-0835 1522-2683 |
DOI: | 10.1002/1522-2683(20000701)21:12<2432::AID-ELPS2432>3.0.CO;2-E |