Alternative splicing, expression, and gene structure of the septin-like putative proto-oncogene Sint1

Sint1 ( sept9), a murine gene of the septin family, was previously isolated as a putative proto-oncogene involved in T-cell lymphomagenesis. We now present its genomic structure and report on nine exons shared by all identified variants and at least four alternatively spliced 5′ exons. Northern blot...

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Bibliographic Details
Published in:Gene 2002-02, Vol.285 (1), p.79-89
Main Authors: Sørensen, Annette Balle, Warming, Søren, Füchtbauer, Ernst-Martin, Pedersen, Finn Skou
Format: Article
Language:English
Subjects:
Alternative Splicing
Animals
Base Sequence
DNA - chemistry
DNA - genetics
DNA-Binding Proteins - genetics
Embryo, Mammalian - metabolism
Embryonic Development
Exons
Female
Gene Expression
Gene Expression Regulation, Developmental
Genes - genetics
Genes, Synthetic
In Situ Hybridization
Introns
Mice
MLL septin-like fusion
Molecular Sequence Data
Oncogene
Pregnancy
Proto-Oncogenes - genetics
Provirus integration
RNA-Binding Proteins - genetics
Sept9
Sequence Analysis, DNA
Sint1 gene
SL3-3 murine leukemia virus
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Summary:Sint1 ( sept9), a murine gene of the septin family, was previously isolated as a putative proto-oncogene involved in T-cell lymphomagenesis. We now present its genomic structure and report on nine exons shared by all identified variants and at least four alternatively spliced 5′ exons. Northern blot analyses using a Sint1 cDNA probe showed in almost all examined tissues two predominant transcripts of 3 and 4 kb. Exon-specific expression analyses assigned one of the 5′ exons to the 4 kb transcript, while the other 5′ exons seem to represent novel, tissue-specific, weakly expressed transcripts of different sizes, and none of them appear to hybridize to the major 3 kb transcript. Whole-mount in situ hybridization on post-implantation embryos revealed several areas strongly expressing Sint1, including neural crest cells, cephalic mesenchyme, and mesenchymal cells in the developing limb. A clustering of proviruses in four independent retrovirally induced tumors point to a region of about 3 kb around the most upstream exon as important for proviral deregulation of Sint1.
ISSN:0378-1119
1879-0038
DOI:10.1016/S0378-1119(02)00406-7