The morphological development of neurons derived from EGF- and FGF-2-driven human CNS precursors depends on their site of integration in the neonatal rat brain

Neural precursor cells derived from the developing human brain were expanded in vitro under the influence of fibroblast growth factor‐2 (FGF‐2) and epidermal growth hormone (EGF), and were then transplanted into different regions of the neonatal rat brain. Four weeks later neurons were seen to have...

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Bibliographic Details
Published in:The European journal of neuroscience 2000-07, Vol.12 (7), p.2405-2413
Main Authors: Rosser, Anne E., Tyers, Pamela, Dunnett, Stephen B.
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Astrocytes - cytology
Astrocytes - transplantation
Brain - cytology
Brain - surgery
Brain Tissue Transplantation
Cell Differentiation - drug effects
Cell Differentiation - physiology
Cell Movement - physiology
Cell Survival - physiology
Epidermal Growth Factor - pharmacology
Fetal Tissue Transplantation
Fetus - cytology
Fibroblast Growth Factor 2 - pharmacology
Humans
neural progenitors
neural stem cells
neural transplantation
neuronal differentiation
Neurons - cytology
Neurons - transplantation
Rats
Species Specificity
Stem Cell Transplantation
Stem Cells - cytology
tau Proteins - analysis
tau Proteins - immunology
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Summary:Neural precursor cells derived from the developing human brain were expanded in vitro under the influence of fibroblast growth factor‐2 (FGF‐2) and epidermal growth hormone (EGF), and were then transplanted into different regions of the neonatal rat brain. Four weeks later neurons were seen to have developed from human embryonic precursors, using a human‐specific antibody to tau (htau). There were morphological differences between implanted neurons developing in the hippocampus, striatum and neocortex, which were confirmed by cell volume measurements, although no specific neurochemical phenotypes were identified. Htau‐positive fibres were seen to project extensively along fibre pathways appropriate for the site of neuronal integration. This study demonstrates that, following cell division in vitro, neurons differentiating from human precursor cell populations retain the ability to respond appropriately to regional determinants present in the neonatal rat brain. This is important for the application of such cells in CNS repair strategies, in particular neural transplantation.
ISSN:0953-816X
1460-9568
DOI:10.1046/j.1460-9568.2000.00135.x