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Cytokines production of U5A2‐13‐positive T cells by stimulation with glycolipid α‐galactosylceramide
We have previously established and reported a novel monoclonal antibody (mAb), U5A2‐13, which recognizes a phenotypically similar population of natural killer (NK)‐like T cells. Using U5A2‐13 mAb, we now describe the functional properties of U5A2‐13+ T cells in both NK1.1‐positive or ‐negative mouse...
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| Published in: | European journal of immunology 2000-08, Vol.30 (8), p.2138-2146 |
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| Main Authors: | , , , , , , , |
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| Language: | English |
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| container_end_page | 2146 |
| container_issue | 8 |
| container_start_page | 2138 |
| container_title | European journal of immunology |
| container_volume | 30 |
| creator | Azuma, Masato Kato, Kazunori Ikarashi, Yoshinori Asada‐Mikami, Rumiko Maruoka, Hidenori Takaue, Yoichi Saito, Atsushi Wakasugi, Hiro |
| description | We have previously established and reported a novel monoclonal antibody (mAb), U5A2‐13, which recognizes a phenotypically similar population of natural killer (NK)‐like T cells. Using U5A2‐13 mAb, we now describe the functional properties of U5A2‐13+ T cells in both NK1.1‐positive or ‐negative mouse strains. Similar to NK1.1+ T cells, hepatic U5A2‐13+ T cells of C57BL/6 (NK1.1+ strain) mice, but not U5A2‐13– T cells, could be induced to produce large amounts of IL‐4 and IFN‐γ by stimulation with glycolipid α‐galactosylceramide (α‐GalCer) present on dendritic cells (DC) in a dose‐dependent manner. The abundant production of these cytokines from U5A2‐13+ T cells of BALB/c (NK1.1– strain) mice is similar to that noted in C57BL/6 mice. Cytokine production by cultures stimulated with DC of β2‐microglobulin‐deficient mice was significantly less than that of cultures stimulated with DC of intact mice. Overall, U5A2‐13+ T cells recognize α‐GalCer presented by CD1d, indicating that U5A2‐13+ T cells can be used to analyze NK‐like T cell function in various strains of mice. |
| doi_str_mv | 10.1002/1521-4141(2000)30:8<2138::AID-IMMU2138>3.0.CO;2-Y |
| format | article |
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Using U5A2‐13 mAb, we now describe the functional properties of U5A2‐13+ T cells in both NK1.1‐positive or ‐negative mouse strains. Similar to NK1.1+ T cells, hepatic U5A2‐13+ T cells of C57BL/6 (NK1.1+ strain) mice, but not U5A2‐13– T cells, could be induced to produce large amounts of IL‐4 and IFN‐γ by stimulation with glycolipid α‐galactosylceramide (α‐GalCer) present on dendritic cells (DC) in a dose‐dependent manner. The abundant production of these cytokines from U5A2‐13+ T cells of BALB/c (NK1.1– strain) mice is similar to that noted in C57BL/6 mice. Cytokine production by cultures stimulated with DC of β2‐microglobulin‐deficient mice was significantly less than that of cultures stimulated with DC of intact mice. Overall, U5A2‐13+ T cells recognize α‐GalCer presented by CD1d, indicating that U5A2‐13+ T cells can be used to analyze NK‐like T cell function in various strains of mice.</description><identifier>ISSN: 0014-2980</identifier><identifier>EISSN: 1521-4141</identifier><identifier>DOI: 10.1002/1521-4141(2000)30:8<2138::AID-IMMU2138>3.0.CO;2-Y</identifier><identifier>PMID: 10940904</identifier><language>eng</language><publisher>Weinheim: WILEY‐VCH Verlag GmbH</publisher><subject>Animals ; Antibodies, Monoclonal - immunology ; Antigens - analysis ; Antigens, CD1 - physiology ; Antigens, CD1d ; Antigens, Ly ; Antigens, Surface ; Cytokines - biosynthesis ; Female ; Galactosylceramides - pharmacology ; glycolipid ^a-galactosylceramide ; Interleukin-4 - physiology ; Killer Cells, Natural - metabolism ; Lectins, C-Type ; Liver - metabolism ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; NK Cell Lectin-Like Receptor Subfamily B ; NK1.1 ; NK‐like T cell ; Proteins - analysis ; U5A2‐13 mAb</subject><ispartof>European journal of immunology, 2000-08, Vol.30 (8), p.2138-2146</ispartof><rights>2000 WILEY‐VCH Verlag GmbH, Weinheim, Fed. 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Using U5A2‐13 mAb, we now describe the functional properties of U5A2‐13+ T cells in both NK1.1‐positive or ‐negative mouse strains. Similar to NK1.1+ T cells, hepatic U5A2‐13+ T cells of C57BL/6 (NK1.1+ strain) mice, but not U5A2‐13– T cells, could be induced to produce large amounts of IL‐4 and IFN‐γ by stimulation with glycolipid α‐galactosylceramide (α‐GalCer) present on dendritic cells (DC) in a dose‐dependent manner. The abundant production of these cytokines from U5A2‐13+ T cells of BALB/c (NK1.1– strain) mice is similar to that noted in C57BL/6 mice. Cytokine production by cultures stimulated with DC of β2‐microglobulin‐deficient mice was significantly less than that of cultures stimulated with DC of intact mice. Overall, U5A2‐13+ T cells recognize α‐GalCer presented by CD1d, indicating that U5A2‐13+ T cells can be used to analyze NK‐like T cell function in various strains of mice.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigens - analysis</subject><subject>Antigens, CD1 - physiology</subject><subject>Antigens, CD1d</subject><subject>Antigens, Ly</subject><subject>Antigens, Surface</subject><subject>Cytokines - biosynthesis</subject><subject>Female</subject><subject>Galactosylceramides - pharmacology</subject><subject>glycolipid ^a-galactosylceramide</subject><subject>Interleukin-4 - physiology</subject><subject>Killer Cells, Natural - metabolism</subject><subject>Lectins, C-Type</subject><subject>Liver - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>NK Cell Lectin-Like Receptor Subfamily B</subject><subject>NK1.1</subject><subject>NK‐like T cell</subject><subject>Proteins - analysis</subject><subject>U5A2‐13 mAb</subject><issn>0014-2980</issn><issn>1521-4141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNqNkU9u1DAUxi0EokPhCsgrRBeZPtvJ2B5QpVGAMlKrEVIHqasnj-MU02QyxAlVdhyBq3ARDsFJcJhSsUGw8T_93vf5vY8QzWDKAPgxyzhLUpay5xwAjgTM1UvOhJrPF8tXyfL8fD3eTsQUpvnqBU8u75HJXc19MgFgacK1ggPyKISPUUPPMv2QHDDQKWhIJ-Q6H7rm2m9doLu2KXrb-WZLm5KuswX_8eUrE3HZNcF3_rOjF9S6qgp0M9DQ-bqvzC_8xncf6FU12KbyO1_Q799i0ZWpjO2aMFTWtab2hXtMHpSmCu7J7X5I1m9eX-Rvk7PV6TJfnCVWaKYSO5Oltkbp2JzUVhgpBbOKZVooXs5EZlMlrSrACMiEMUypjRSxIakzbePxkDzb68aOPvUudFj7MH7cbF3TB5RMznjK_w1GTnHJWATf7UHbNiG0rsRd62vTDsgAx6hwHDuOY8cxKhSACsdwEGNU-DsqFAiYr5DjZdR8emveb2pX_KG4zyYC7_fAja_c8P-OfzG8exM_Af4nsDE</recordid><startdate>20000801</startdate><enddate>20000801</enddate><creator>Azuma, Masato</creator><creator>Kato, Kazunori</creator><creator>Ikarashi, Yoshinori</creator><creator>Asada‐Mikami, Rumiko</creator><creator>Maruoka, Hidenori</creator><creator>Takaue, Yoichi</creator><creator>Saito, Atsushi</creator><creator>Wakasugi, Hiro</creator><general>WILEY‐VCH Verlag GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20000801</creationdate><title>Cytokines production of U5A2‐13‐positive T cells by stimulation with glycolipid α‐galactosylceramide</title><author>Azuma, Masato ; Kato, Kazunori ; Ikarashi, Yoshinori ; Asada‐Mikami, Rumiko ; Maruoka, Hidenori ; Takaue, Yoichi ; Saito, Atsushi ; Wakasugi, Hiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3918-c67f9ca8921379c3a7731c8159382f635c487c8d0a3053aa188b739407959cb73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antigens - analysis</topic><topic>Antigens, CD1 - physiology</topic><topic>Antigens, CD1d</topic><topic>Antigens, Ly</topic><topic>Antigens, Surface</topic><topic>Cytokines - biosynthesis</topic><topic>Female</topic><topic>Galactosylceramides - pharmacology</topic><topic>glycolipid ^a-galactosylceramide</topic><topic>Interleukin-4 - physiology</topic><topic>Killer Cells, Natural - metabolism</topic><topic>Lectins, C-Type</topic><topic>Liver - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>NK Cell Lectin-Like Receptor Subfamily B</topic><topic>NK1.1</topic><topic>NK‐like T cell</topic><topic>Proteins - analysis</topic><topic>U5A2‐13 mAb</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Azuma, Masato</creatorcontrib><creatorcontrib>Kato, Kazunori</creatorcontrib><creatorcontrib>Ikarashi, Yoshinori</creatorcontrib><creatorcontrib>Asada‐Mikami, Rumiko</creatorcontrib><creatorcontrib>Maruoka, Hidenori</creatorcontrib><creatorcontrib>Takaue, Yoichi</creatorcontrib><creatorcontrib>Saito, Atsushi</creatorcontrib><creatorcontrib>Wakasugi, Hiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Azuma, Masato</au><au>Kato, Kazunori</au><au>Ikarashi, Yoshinori</au><au>Asada‐Mikami, Rumiko</au><au>Maruoka, Hidenori</au><au>Takaue, Yoichi</au><au>Saito, Atsushi</au><au>Wakasugi, Hiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytokines production of U5A2‐13‐positive T cells by stimulation with glycolipid α‐galactosylceramide</atitle><jtitle>European journal of immunology</jtitle><addtitle>Eur J Immunol</addtitle><date>2000-08-01</date><risdate>2000</risdate><volume>30</volume><issue>8</issue><spage>2138</spage><epage>2146</epage><pages>2138-2146</pages><issn>0014-2980</issn><eissn>1521-4141</eissn><abstract>We have previously established and reported a novel monoclonal antibody (mAb), U5A2‐13, which recognizes a phenotypically similar population of natural killer (NK)‐like T cells. Using U5A2‐13 mAb, we now describe the functional properties of U5A2‐13+ T cells in both NK1.1‐positive or ‐negative mouse strains. Similar to NK1.1+ T cells, hepatic U5A2‐13+ T cells of C57BL/6 (NK1.1+ strain) mice, but not U5A2‐13– T cells, could be induced to produce large amounts of IL‐4 and IFN‐γ by stimulation with glycolipid α‐galactosylceramide (α‐GalCer) present on dendritic cells (DC) in a dose‐dependent manner. The abundant production of these cytokines from U5A2‐13+ T cells of BALB/c (NK1.1– strain) mice is similar to that noted in C57BL/6 mice. Cytokine production by cultures stimulated with DC of β2‐microglobulin‐deficient mice was significantly less than that of cultures stimulated with DC of intact mice. Overall, U5A2‐13+ T cells recognize α‐GalCer presented by CD1d, indicating that U5A2‐13+ T cells can be used to analyze NK‐like T cell function in various strains of mice.</abstract><cop>Weinheim</cop><pub>WILEY‐VCH Verlag GmbH</pub><pmid>10940904</pmid><doi>10.1002/1521-4141(2000)30:8<2138::AID-IMMU2138>3.0.CO;2-Y</doi><tpages>10</tpages></addata></record> |
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| ispartof | European journal of immunology, 2000-08, Vol.30 (8), p.2138-2146 |
| issn | 0014-2980 1521-4141 |
| language | eng |
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| source | Wiley-Blackwell Read & Publish Collection |
| subjects | Animals Antibodies, Monoclonal - immunology Antigens - analysis Antigens, CD1 - physiology Antigens, CD1d Antigens, Ly Antigens, Surface Cytokines - biosynthesis Female Galactosylceramides - pharmacology glycolipid ^a-galactosylceramide Interleukin-4 - physiology Killer Cells, Natural - metabolism Lectins, C-Type Liver - metabolism Mice Mice, Inbred BALB C Mice, Inbred C57BL NK Cell Lectin-Like Receptor Subfamily B NK1.1 NK‐like T cell Proteins - analysis U5A2‐13 mAb |
| title | Cytokines production of U5A2‐13‐positive T cells by stimulation with glycolipid α‐galactosylceramide |
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