Cytokines production of U5A2‐13‐positive T cells by stimulation with glycolipid α‐galactosylceramide

We have previously established and reported a novel monoclonal antibody (mAb), U5A2‐13, which recognizes a phenotypically similar population of natural killer (NK)‐like T cells. Using U5A2‐13 mAb, we now describe the functional properties of U5A2‐13+ T cells in both NK1.1‐positive or ‐negative mouse...

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Bibliographic Details
Published in:European journal of immunology 2000-08, Vol.30 (8), p.2138-2146
Main Authors: Azuma, Masato, Kato, Kazunori, Ikarashi, Yoshinori, Asada‐Mikami, Rumiko, Maruoka, Hidenori, Takaue, Yoichi, Saito, Atsushi, Wakasugi, Hiro
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - immunology
Antigens - analysis
Antigens, CD1 - physiology
Antigens, CD1d
Antigens, Ly
Antigens, Surface
Cytokines - biosynthesis
Female
Galactosylceramides - pharmacology
glycolipid ^a-galactosylceramide
Interleukin-4 - physiology
Killer Cells, Natural - metabolism
Lectins, C-Type
Liver - metabolism
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
NK Cell Lectin-Like Receptor Subfamily B
NK1.1
NK‐like T cell
Proteins - analysis
U5A2‐13 mAb
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Summary:We have previously established and reported a novel monoclonal antibody (mAb), U5A2‐13, which recognizes a phenotypically similar population of natural killer (NK)‐like T cells. Using U5A2‐13 mAb, we now describe the functional properties of U5A2‐13+ T cells in both NK1.1‐positive or ‐negative mouse strains. Similar to NK1.1+ T cells, hepatic U5A2‐13+ T cells of C57BL/6 (NK1.1+ strain) mice, but not U5A2‐13– T cells, could be induced to produce large amounts of IL‐4 and IFN‐γ by stimulation with glycolipid α‐galactosylceramide (α‐GalCer) present on dendritic cells (DC) in a dose‐dependent manner. The abundant production of these cytokines from U5A2‐13+ T cells of BALB/c (NK1.1– strain) mice is similar to that noted in C57BL/6 mice. Cytokine production by cultures stimulated with DC of β2‐microglobulin‐deficient mice was significantly less than that of cultures stimulated with DC of intact mice. Overall, U5A2‐13+ T cells recognize α‐GalCer presented by CD1d, indicating that U5A2‐13+ T cells can be used to analyze NK‐like T cell function in various strains of mice.
ISSN:0014-2980
1521-4141
DOI:10.1002/1521-4141(2000)30:8<2138::AID-IMMU2138>3.0.CO;2-Y