Dietary Fish Oil Reduces Microvascular Thrombosis in a Porcine Experimental Model

Microvascular thrombosis plays a significant role in the pathophysiology of ischaemic reperfusion injury. A fish oil-supplemented diet containing n-3 polyunsaturated fatty acids (PUFA) reduces thromboxane A 2 (TxA 2) synthesis and, thus, vasoconstriction and platelet aggregation. The aim of this stu...

Full description

Saved in:
Bibliographic Details
Published in:Thrombosis research 2000-07, Vol.99 (2), p.203-208
Main Authors: Thorwest, Martin, Balling, Eva, Kristensen, Steen D, Aagaard, Susanne, Hakami, Ardeshir, Husted, Steen E, Marqversen, Jørgen, Hjortdal, Vibeke E
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Bleeding Time
Dietary Fats, Unsaturated - pharmacology
Dietary fish oil
Disease Models, Animal
Diseases of the cardiovascular system
Fatty Acids, Omega-3 - pharmacology
Fibrinogen - drug effects
Fibrinogen - metabolism
Fish Oils - pharmacology
Medical sciences
Microcirculation - physiopathology
Microvascular thrombosis
n-3 PUFA
Platelet Count - drug effects
Porcine
Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)
Reperfusion Injury - blood
Reperfusion Injury - pathology
Swine
Thrombosis - prevention & control
Thromboxane B2 - blood
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Microvascular thrombosis plays a significant role in the pathophysiology of ischaemic reperfusion injury. A fish oil-supplemented diet containing n-3 polyunsaturated fatty acids (PUFA) reduces thromboxane A 2 (TxA 2) synthesis and, thus, vasoconstriction and platelet aggregation. The aim of this study was to elucidate whether n-3 PUFA in a porcine model of ischaemia and reperfusion injury 1) inhibit accumulation of platelets and fibrinogen in ischaemia-reperfusion injured tissue, 2) prolong the bleeding time, and 3) inhibit TxA 2 synthesis. Nine pigs were fed a standard diet supplemented with 7 g n-3 PUFA/day for 3 weeks. Nine pigs on the standard diet served as controls. Unilateral myocutaneous flaps were exposed to ischaemia for a period of 6 hours. Contralateral flaps were nonischaemic. Tissue contents of radioactive-labelled platelets and fibrinogen were measured after 4 hours of reperfusion. Platelet count, serum TxB 2, and the cutaneous bleeding time were measured before and after 3 weeks of diet. In the fish oil group, the accumulation of platelets was significantly reduced in all the myocutaneous flaps, except in the ischaemic skin part, when compared to control animals. Fibrinogen was significantly reduced in nonischaemic flaps, but not in ischaemic flaps. After the feeding period, the level of TxB 2 was significantly lowered in the fish oil group ( p
ISSN:0049-3848
1879-2472
DOI:10.1016/S0049-3848(00)00233-4