role of lipoxygenase products on the endocytosis of yolk proteins in insects: participation of cAMP

The participation of eicosanoids and second messengers in the regulation of endocytosis by the ovaries was investigated using the uptake of Rhodnius heme binding protein (RHBP) as an experimental model. The rate of RHBP uptake decreased up to 40% in the presence of BWA4C and NDGA, 5 and 12-lipoxygen...

Full description

Saved in:
Bibliographic Details
Published in:Archives of insect biochemistry and physiology 2004-04, Vol.55 (4), p.178-187
Main Authors: Medeiros, M.N, Mendonca, L.H, Hunter, A.L, Paiva-Silva, G.O, Mello, F.G, Henze, I.P, Masuda, H, Maya-Monteiro, C.M, Machado, E.A
Format: Article
Language:English
Subjects:
Animals
binding proteins
cAMP
Carrier Proteins - metabolism
cyclic AMP
Cyclic AMP - metabolism
dibutyryl cyclic AMP
Egg Proteins - metabolism
eicosanoid synthesis inhibitors
eicosanoids
Eicosanoids - physiology
endocytosis
Endocytosis - physiology
enzyme inhibitors
Enzyme Inhibitors - pharmacology
Female
heme
heme binding protein
Hemeproteins - metabolism
indomethacin
insect biochemistry
leukotriene
leukotrienes
lipoxygenase
Lipoxygenase - drug effects
Lipoxygenase - metabolism
Models, Biological
oogenesis
ovaries
Ovary - metabolism
prostaglandin
prostaglandin synthase
prostaglandins
Reduviidae
Rhodnius
Rhodnius - metabolism
Rhodnius prolixus
Second Messenger Systems
second messengers
uptake mechanisms
yolk proteins
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The participation of eicosanoids and second messengers in the regulation of endocytosis by the ovaries was investigated using the uptake of Rhodnius heme binding protein (RHBP) as an experimental model. The rate of RHBP uptake decreased up to 40% in the presence of BWA4C and NDGA, 5 and 12-lipoxygenase inhibitors, respectively, suggesting the involvement of lipoxygenase products in endocytosis regulation. Addition of Leukotriene B4 (LTB4; one product of the 5 lipoxygenase pathway) increased in vitro the uptake of RHBP by 30%. The content of cAMP in the Rhodnius' ovaries were monitored after treatment with different eicosanoids and inhibitors of eicosanoids synthesis. The amount of cAMP decreased in the presence of indomethacin (by 50%), while treatment with PGE2 induced an increase of 85% of this messenger in the ovaries. The presence of LTB4 in the medium inhibited in 60% the content of cAMP in the ovaries, while BWA4C induced a 100% increase of this messenger in the ovaries. Addition of 1 micromolar DBcAMP in the medium resulted in a 30% decrease in the rate of RHBP uptake. Taken together, these data show that cyclooxygenase and lipoxygenase products participate in the control of protein internalization by modulation of cAMP levels.
ISSN:0739-4462
1520-6327
DOI:10.1002/arch.10129