Genomic Construct and Mapping of the Gene for CMAP (Leukocystatin/Cystatin F, CST7) and Identification of a Proximal Novel Gene, BSCv (C20orf3)

It is proposed that CMAP (leukocystatin/cystatin F, HGMW-approved symbol CST7) expression is correlated with the metastatic potential of malignant tumors. FISH analysis of human and murine CMAP revealed the genomic loci 20p11.21–p11.22 of the human family 2 cystatin cluster and mouse chromosome regi...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 2000-07, Vol.67 (1), p.87-91
Main Authors: Morita, Masashi, Hara, Yoshikazu, Tamai, Yoshitaka, Arakawa, Hiroharu, Nishimura, Susumu
Format: Article
Language:English
Subjects:
Alleles
Amino Acid Sequence
Animals
Biological and medical sciences
Biomarkers, Tumor
Chromosome Mapping
Chromosomes, Human, Pair 2
Classical genetics, quantitative genetics, hybrids
Computational Biology
Cystatins - genetics
DNA Primers - chemistry
DNA, Neoplasm - analysis
DNA, Neoplasm - genetics
Exons
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Human
Humans
In Situ Hybridization, Fluorescence
Introns
Mice
Molecular Sequence Data
Sequence Homology, Amino Acid
Tandem Repeat Sequences
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Summary:It is proposed that CMAP (leukocystatin/cystatin F, HGMW-approved symbol CST7) expression is correlated with the metastatic potential of malignant tumors. FISH analysis of human and murine CMAP revealed the genomic loci 20p11.21–p11.22 of the human family 2 cystatin cluster and mouse chromosome region 2G1–G3, respectively. Like murine CMAP, the human CMAP gene is constructed from four divided exons, all of which encode the functional domains of the putative translational product. Based on the computational analysis, a novel gene weakly similar to the plant strictosidine synthase, named BSCv (HGMW-approved symbol C20orf3), was identified on the opposite allele at a distance of a few kilobases from the human CMAP gene. In between human CMAP and the BSCv gene, there is a unique tandem repeat sequence. CpG-rich island characteristics and GC-box features normally observed in housekeeping genes were not seen around exon 1 of the CMAP gene, reflecting the restricted expression of CMAP in hematopoietic cells.
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.2000.6237