Trivalent Ions Activate Abscisic Acid-Inducible Promoters through an ABI1-Dependent Pathway in Rice Protoplasts

The plant hormone abscisic acid (ABA) mediates many vital processes in plant growth and development, including seed dormancy, cell division, water use efficiency, and adaptation to drought, salinity, chilling, pathogen attack, and UV light. Our understanding of ABA signal transduction is fragmentary...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 2000-08, Vol.123 (4), p.1553-1560
Main Authors: Dik Hagenbeek, Quatrano, Ralph S., Rock, Christopher D.
Format: Article
Language:English
Subjects:
ABI1 gene
Abscisic Acid - metabolism
Arabidopsis - genetics
Arabidopsis Proteins
Biological and medical sciences
Calcium
Calcium Channel Blockers - pharmacology
Cations - pharmacology
Cell Biology and Signal Transduction
Cell physiology
Complementary DNA
Cytometry
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Gene expression
Genes, Reporter
Glucuronidase - genetics
Glucuronidase - metabolism
Green Fluorescent Proteins
Hordeum - genetics
Hordeum vulgare
HVA1 gene
Lanthanum
Luciferases - genetics
Luciferases - metabolism
Luminescent Proteins - genetics
Luminescent Proteins - metabolism
Metals, Rare Earth - pharmacology
Molecular and cellular biology
Oryza - genetics
Oryza - metabolism
Oryza sativa
Phosphoprotein Phosphatases - genetics
Phosphoprotein Phosphatases - metabolism
Plant Proteins
Plants
Plasmids
Promoter Regions, Genetic
Protoplasts
Protoplasts - metabolism
Rice
Signal Transduction
Space life sciences
Trans-Activators
Transcription Factors - genetics
Transcription Factors - metabolism
Transcriptional Activation
VIVIPAROUS1 gene
Zea mays
Zea mays - genetics
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Summary:The plant hormone abscisic acid (ABA) mediates many vital processes in plant growth and development, including seed dormancy, cell division, water use efficiency, and adaptation to drought, salinity, chilling, pathogen attack, and UV light. Our understanding of ABA signal transduction is fragmentary and would benefit from specific and facile probes of the process. Protoplasts from rice (Oryza sativa L. cv IR54) embryonic suspension cultures cotransformed with effector plasmids encoding the maize (Zea mays) VIVIPAROUS1 cDNA and/or the Arabidopsis dominant negative mutant (abi1-1) ABA-insensitive cDNA demonstrated genetic interactions of VIVIPAROUS1 and abil-1 in transactivation of the ABA-inducible HVA1 promoter from barley (Hordeum vulgare), suggesting the mechanisms of these effectors are conserved among monocots and dicots. Trivalent ions have been shown to act as an effector of gene expression in plants and animals, although the mechanism of action is unknown. We show in two complementary transient ABA-inducible gene expression assays (β-glucuronidase and luciferase enzymatic activities and quantitative flow cytometry of green fluorescent protein) that trivalent ions specifically interact with an ABI1-dependent ABA-signaling pathway leading to gene expression. Trivalent ions mimic ABA effects on gene expression and may be a useful tool to study ABA signaling.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.123.4.1553