Circular dichroism spectroscopy reveals invariant conformation of guanine runs in DNA

We demonstrate that the characteristic circular dichroism (CD) features of the parallel‐stranded DNA tetraplex of d(G4), especially the strong band at 260 nm, are characteristic for the B and A forms of the antiparallel duplex of d(C4G4). Hence, this band evidently originates from intrastrand guanin...

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Bibliographic Details
Published in:Biopolymers 2002, Vol.67 (4-5), p.275-277
Main Authors: Kypr, Jaroslav, Vorlíčková, Michaela
Format: Article
Language:English
Subjects:
Circular Dichroism
Dimerization
DNA
DNA - chemistry
guanine
Guanine - chemistry
Nucleic Acid Conformation
single strand
spectroscopy
tetraplex
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Summary:We demonstrate that the characteristic circular dichroism (CD) features of the parallel‐stranded DNA tetraplex of d(G4), especially the strong band at 260 nm, are characteristic for the B and A forms of the antiparallel duplex of d(C4G4). Hence, this band evidently originates from intrastrand guanine–guanine stacking, which is therefore very similar in the duplex and tetraplex DNA. In addition, the same type of the CD spectrum is provided by the ordered single strand of d(GA)10. This observation suggests that the ordered single strand of d(GA)10 is stabilized by a core of guanines stacked like in the parallel tetraplex. This view is used to start the modeling of the molecular structure of the ordered d(GA)10 single strand. Our studies suggest that guanine itself is strong enough to stabilize various secondary structures of DNA, which is a property relevant to thinking about the origin and evolution of molecular replicators. © 2002 Wiley Periodicals, Inc. Biopolymers (Biospectroscopy) 67: 275–277, 2002
ISSN:0006-3525
1097-0282
DOI:10.1002/bip.10112