Effects of prolactin on intracellular calcium concentration and cell proliferation in human glioma cells

Prolactin (PRL) has several physiological effects on peripheral tissues and the brain. This hormone acts via its membrane receptor (PRL‐R) to induce cell differentiation or proliferation. Using reverse transcription–polymerase chain reaction (RT‐PCR) combined with Southern blot analysis, we detected...

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Bibliographic Details
Published in:Glia 2002-05, Vol.38 (3), p.200-214
Main Authors: Ducret, Thomas, Boudina, Sihem, Sorin, Bruno, Vacher, Anne Marie, Gourdou, Isabelle, Liguoro, Dominique, Guerin, Jean, Bresson-Bepoldin, Laurence, Vacher, Pierre
Format: Article
Language:English
Subjects:
astrocyte
Biological and medical sciences
Brain Neoplasms - metabolism
Calcium - metabolism
calcium imaging
calcium influx
Calcium Signaling - drug effects
Calcium Signaling - physiology
calcium wave
Calcium-Transporting ATPases - antagonists & inhibitors
Calcium-Transporting ATPases - metabolism
Cell Differentiation - drug effects
Cell Differentiation - physiology
Cell Division - drug effects
Cell Division - physiology
Cell Nucleus - drug effects
Cell Nucleus - metabolism
Cytosol - drug effects
Cytosol - metabolism
DNA - biosynthesis
DNA - drug effects
Dose-Response Relationship, Drug
Extracellular Space - drug effects
Extracellular Space - metabolism
Female
Fundamental and applied biological sciences. Psychology
Glioma - metabolism
Humans
Intracellular Fluid - drug effects
Intracellular Fluid - metabolism
Isolated neuron and nerve. Neuroglia
Janus Kinase 2
Male
microspectrofluorimetry
nuclear calcium
Prolactin - metabolism
Prolactin - pharmacology
prolactin receptor
Protein-Tyrosine Kinases - antagonists & inhibitors
Protein-Tyrosine Kinases - metabolism
Proto-Oncogene Proteins
Receptors, Prolactin - agonists
Receptors, Prolactin - genetics
Receptors, Prolactin - metabolism
RNA, Messenger - metabolism
RT-PCR
Tumor Cells, Cultured
tyrosine kinase
Vertebrates: nervous system and sense organs
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Summary:Prolactin (PRL) has several physiological effects on peripheral tissues and the brain. This hormone acts via its membrane receptor (PRL‐R) to induce cell differentiation or proliferation. Using reverse transcription–polymerase chain reaction (RT‐PCR) combined with Southern blot analysis, we detected PRL‐R transcripts in a human glioma cell line (U87‐MG) and in primary cultured human glioblastoma cells. These transcripts were deleted or not in their extracellular domains. We examined the effects of PRL on intracellular free Ca2+ concentration ([Ca2+]i) in these cells in order to improve our understanding of the PRL transduction mechanism, which is still poorly documented. [Ca2+]i was measured by microspectrofluorimetry using indo‐1 as the Ca2+ fluorescent probe. Spatiotemporal aspects of PRL‐induced Ca2+ signals were investigated using high‐speed fluo‐3 confocal imaging. We found that physiological concentrations (0.4–4 nM) of PRL‐stimulated Ca2+ entry and intracellular Ca2+ mobilization via a tyrosine kinase–dependent mechanism. The two types of Ca2+ responses observed were distinguishable by their kinetics: one showing a slow (type I) and the other a fast (type II) increase in [Ca2+]i. The amplitude of PRL‐induced Ca2+ increases may be sufficient to provoke several physiological responses, such as stimulating proliferation. Furthermore, PRL induced a dose‐dependent increase in [3H]thymidine incorporation levels and in cellular growth and survival, detected by the MTT method. These data indicate that PRL induced mitogenesis of human glioma cells. GLIA 38:200–214, 2002. © 2002 Wiley‐Liss, Inc.
ISSN:0894-1491
1098-1136
DOI:10.1002/glia.10056