The fifth gene of the iol operon of Bacillus subtilis, iolE, encodes 2-keto-myo-inositol dehydratase

1 Department of Biotechnology, Faculty of Life Science and Biotechnology, Fukuyama University, 985 Sanzo, Higashimura-cho, Fukuyama-shi, Hiroshima 729-0292, Japan 2 Central Research Laboratories, Hokko Chemical Industry Co., Ltd, 2165 Toda, Atsugi-shi, Kanagawa 243-0023, Japan 3 Department of Enviro...

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Bibliographic Details
Published in:Microbiology (Society for General Microbiology) 2004-03, Vol.150 (3), p.571-580
Main Authors: Yoshida, Ken-ichi, Yamaguchi, Masanori, Ikeda, Hideki, Omae, Kaoru, Tsurusaki, Ken-ichi, Fujita, Yasutaro
Format: Article
Language:English
Subjects:
Bacillus subtilis
Bacillus subtilis - enzymology
Bacillus subtilis - genetics
Base Sequence
DNA, Bacterial - genetics
Escherichia coli - genetics
Genes, Bacterial
Hydro-Lyases - genetics
Hydro-Lyases - metabolism
Inositol - analogs & derivatives
Inositol - metabolism
Operon
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Summary:1 Department of Biotechnology, Faculty of Life Science and Biotechnology, Fukuyama University, 985 Sanzo, Higashimura-cho, Fukuyama-shi, Hiroshima 729-0292, Japan 2 Central Research Laboratories, Hokko Chemical Industry Co., Ltd, 2165 Toda, Atsugi-shi, Kanagawa 243-0023, Japan 3 Department of Environment and Information Science, Faculty of Human Culture and Sciences, Fukuyama University, 985 Sanzo, Higashimura-cho, Fukuyama-shi, Hiroshima 729-0292, Japan Correspondence Ken-ichi Yoshida kyoshida{at}bt.fubt.fukuyama-u.ac.jp The myo -inositol catabolism pathway of Bacillus subtilis has not been fully characterized but was proposed to involve step-wise multiple reactions that finally yielded acetyl-CoA and dihydroxyacetone phosphate. It is known that the iolABCDEFGHIJ operon is responsible for the catabolism of inositol. IolG catalyses the first step of myo -inositol catabolism, the dehydrogenation of myo -inositol, producing 2-keto- myo -inositol (inosose). The second step was thought to be the dehydration of inosose. Genetic and biochemical analyses of the iol genes led to the identification of iolE , encoding the enzyme for the second step of inositol catabolism, inosose dehydratase. The reaction product of inosose dehydratase was identified as D -2,3-diketo-4-deoxy- epi -inositol. Abbreviations: ESI-TOF, electrospray ionization-time-of-flight
ISSN:1350-0872
1465-2080
DOI:10.1099/mic.0.26768-0