Weaning Is Associated with an Upregulation of Expression of Inflammatory Cytokines in the Intestine of Piglets

Cytokines play a central role in immune cell response, but they also participate in the maintenance of tissue integrity. Changes in the cytokine network of the pig gut may be expected at weaning, because abrupt changes in dietary and environmental factors lead to important morphological and function...

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Published in:The Journal of nutrition 2004-03, Vol.134 (3), p.641-647
Main Authors: Lallès, J.P., Sève, B., Pié, S., Blazy, F., Laffitte, J., Oswald, I.P.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
cytokines
Cytokines - genetics
DNA Primers
Feeding. Feeding behavior
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation - immunology
Immunity, Mucosal
Inflammation - genetics
Interleukins - genetics
Intestinal Mucosa - immunology
intestine
Lactase - metabolism
piglets
Reverse Transcriptase Polymerase Chain Reaction
Sucrase - metabolism
Swine
Time Factors
Tumor Necrosis Factor-alpha - genetics
Vertebrates: anatomy and physiology, studies on body, several organs or systems
Weaning
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Summary:Cytokines play a central role in immune cell response, but they also participate in the maintenance of tissue integrity. Changes in the cytokine network of the pig gut may be expected at weaning, because abrupt changes in dietary and environmental factors lead to important morphological and functional adaptations in the gut. This study measured the gene expression of 6 inflammatory cytokines along the small intestine (SI) and the proximal colon in 28-d-old piglets (n = 45) at different time points (0, 1, 2, 5 and 8 d) postweaning, using RT-PCR. Villus-crypt architecture and enzymatic activities of lactase and sucrase in the SI were also examined. The results confirmed that weaning is associated with morphological and enzymatic changes in the SI. In addition, the data indicated that cytokine response in the gut could be divided into two periods: an early acute response (0 to 2 d postweaning) and a late long-lasting response (2 to 8 d postweaning). Between d 0 and d 2, the levels of IL-1β, IL-6, and TNF-α messenger RNA (mRNA) increased. Marked upregulation of IL-1β mRNA occurred in most parts of the intestine, whereas IL-6 and TNF-α mRNA markedly increased only at specific sites in the intestine. Between d 2 and d 8, the levels of IL-1β, IL-6, and TNF-α mRNA rapidly returned to preweaning values, except that the level of TNF-α mRNA remained high in the distal SI. Levels of IL-12 subunit p40 (IL-12p40) and IL-18 mRNA also decreased, compared to those on d 0. Taken together, these results demonstrate that weaning in piglets is associated with an early and transient response in gene expression of inflammatory cytokines in the gut.
ISSN:0022-3166
1541-6100
DOI:10.1093/jn/134.3.641