Cloning and expression of an ABSCISIC ACID‐INSENSITIVE 3 (ABI3) gene homologue of yellow‐cedar (Chamaecyparis nootkatensis)

A homologue of the ABI3 gene was isolated from the conifer species, Chamaecyparis nootkatensis. The deduced protein of 794 amino acids exhibited sequence similarity to other VP1/ABI3 proteins within four regions. Expression occurs exclusively in seeds, with no detectable mRNA in leaves and roots. Un...

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Bibliographic Details
Published in:Journal of experimental botany 2002-05, Vol.53 (371), p.1219-1221
Main Authors: Lazarova, Galina, Zeng, Ying, Kermode, Allison R.
Format: Article
Language:English
Subjects:
ABI3
Abscisic acid
Abscisic Acid - metabolism
Abscisic Acid - pharmacology
Amino Acid Sequence
Amino acids
Angiosperms
Arabidopsis Proteins
Biological and medical sciences
Cloning, Molecular
Complementary DNA
Cupressaceae - genetics
DNA, Complementary - chemistry
DNA, Complementary - genetics
Dormancy
Embryos
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Plant - drug effects
GENE NOTE
Genes
Genes. Genome
Introns
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Plant Proteins - genetics
Polymerase chain reaction
Proteins
RNA
seed dormancy
Seeds - genetics
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Transcription Factors
yellow‐cedar
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Summary:A homologue of the ABI3 gene was isolated from the conifer species, Chamaecyparis nootkatensis. The deduced protein of 794 amino acids exhibited sequence similarity to other VP1/ABI3 proteins within four regions. Expression occurs exclusively in seeds, with no detectable mRNA in leaves and roots. Unlike the homologues of angiosperms, CnABI3 may be encoded by more than one gene.
ISSN:0022-0957
1460-2431
DOI:10.1093/jexbot/53.371.1219