The excretion of F18+ E. coli is reduced after oral immunisation of pigs with a FedF and F4 fimbriae conjugate

Summary Currently, no vaccines are available for edema disease and post-weaning diarrhoea (PWD) in pigs. In the present study, a subunit vaccine containing the F18 fimbrial adhesin FedF was studied. Hereto, recombinant FedF was produced as a fusion protein with maltose-binding protein. Even though t...

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Bibliographic Details
Published in:Vaccine 2008-04, Vol.26 (17), p.2154-2163
Main Authors: Tiels, P, Verdonck, F, Coddens, A, Goddeeris, B, Cox, E
Format: Article
Language:English
Subjects:
Adhesins, Bacterial - immunology
Administration, Oral
Allergy and Immunology
Animals
Applied microbiology
Bacterial Vaccines - administration & dosage
Bacterial Vaccines - immunology
Biological and medical sciences
Diarrhea - prevention & control
Diarrhea - veterinary
Edema Disease of Swine - immunology
Escherichia coli - genetics
Escherichia coli - immunology
Escherichia coli - metabolism
Escherichia coli Infections - prevention & control
Escherichia coli Infections - veterinary
Escherichia coli Proteins - immunology
ETEC
F18 fimbriae
F4 fimbriae
FedF
Fimbriae Proteins - immunology
Fimbriae Proteins - metabolism
Fimbriae, Bacterial - immunology
Fimbriae, Bacterial - metabolism
Fundamental and applied biological sciences. Psychology
Immunization
Microbiology
Pig
Swine
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Vaccines, Conjugate - immunology
VTEC
Weaning
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Summary:Summary Currently, no vaccines are available for edema disease and post-weaning diarrhoea (PWD) in pigs. In the present study, a subunit vaccine containing the F18 fimbrial adhesin FedF was studied. Hereto, recombinant FedF was produced as a fusion protein with maltose-binding protein. Even though the produced MBPFedF was shown to attach in vitro to enterocytes, almost no FedF-specific immune response could be detected after oral administration to piglets. The delivery of FedF to the intestinal mucosa was improved by conjugating the MBPFedF to F4 fimbriae. Indeed, this conjugation induced a systemic and local FedF-specific immune response and led to a reduction in excretion after infection with F18+ E. coli . Although complete protection was not observed, the conjugation between FedF and F4 fimbriae can be considered as a first step towards the development of a combined vaccine against F4+ and F18+ E. coli infections.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2008.01.054