Molecular diversity of the dystrophin-like protein complex in the developing and adult avian retina

Mutations in dystrophin cause muscular dystrophy but also affect the CNS, including information processing in the retina. To better understand the molecular basis of these CNS deficits, we analyzed the molecular composition and developmental appearance of dystrophin and of the dystrophin-associated...

Full description

Saved in:
Bibliographic Details
Published in:Neuroscience 2002-01, Vol.111 (2), p.259-273
Main Authors: Blank, M, Blake, D.J, Kröger, S
Format: Article
Language:English
Subjects:
Aging - metabolism
Animals
Animals, Newborn - metabolism
Animals, Newborn - physiology
Biological and medical sciences
Chick Embryo - metabolism
Chick Embryo - physiology
Chickens - metabolism
Collagenases - administration & dosage
Collagenases - pharmacology
Cytoskeletal Proteins - metabolism
dystrobrevin
dystroglycan
Dystroglycans
Dystrophin - metabolism
Dystrophin-Associated Proteins
extracellular matrix
Extracellular Matrix - physiology
Eye and associated structures. Visual pathways and centers. Vision
Fundamental and applied biological sciences. Psychology
Injections
inner limiting membrane
Membrane Glycoproteins - metabolism
Membrane Proteins - metabolism
Müller glial cell
photoreceptor
Retina - embryology
Retina - growth & development
Retina - metabolism
Retina - physiology
Solubility
Synapses - physiology
Tissue Distribution
Utrophin
Vertebrates: nervous system and sense organs
Vitreous Body - drug effects
Vitreous Body - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Mutations in dystrophin cause muscular dystrophy but also affect the CNS, including information processing in the retina. To better understand the molecular basis of these CNS deficits, we analyzed the molecular composition and developmental appearance of dystrophin and of the dystrophin-associated protein complex (DPC) in the embryonic and adult avian retina. We detected a concentration of the DPC at the vitreal border and in the outer plexiform layer of the adult retina. At both locations the complex had a different molecular composition and different developmental expression pattern. At the vitreal border, the complex was composed of utrophin, α-dystrobrevin-1, and dystroglycan, and was present at all stages of retinal development even before neurogenesis and gliogenesis. On the other hand, the complex in the outer plexiform layer consisted of dystrophin, β-dystrobrevin and dystroglycan. The distribution of this complex changed from a diffusely distributed to an aggregated form during development concomitant with synapse formation in the outer plexiform layer. Solubilization of the retinal extracellular matrix by intravitreal injection of collagenase resulted in a redistribution of the complex at the retinal vitreal border but had no influence on the distribution of the dystrophin-associated proteins in the outer plexiform layer. These results demonstrate two types of dystrophin-like complexes in the chick retina with differential molecular compositions, different anchorage to the extracellular matrix, and different developmental expression patterns, suggesting distinct functions for the DPC at both locations.
ISSN:0306-4522
1873-7544
DOI:10.1016/S0306-4522(02)00032-5