Immobilization of glycoproteins, such as VEGF, on biodegradable substrates

Attachment of growth factors to biodegradable polymers, such as poly(lactide-co-glycolide) (PLGA), may enhance and/or accelerate integration of tissue engineering scaffolds. Although proteins are commonly bound via abundant amino groups, a more selective approach may increase bioactivity of immobili...

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Bibliographic Details
Published in:Acta biomaterialia 2008-07, Vol.4 (4), p.1016-1023
Main Authors: Sharon, J.L., Puleo, D.A.
Format: Article
Language:English
Subjects:
Animals
Carbodiimides - chemistry
Cattle
Cell Proliferation - drug effects
Endothelial Cells - cytology
Endothelial Cells - drug effects
Glycoprotein
Glycoproteins - metabolism
Humans
Hydrazines - chemistry
Hydrazines - metabolism
PLGA
Polyglactin 910 - metabolism
Protein immobilization
Surface Properties
Vascular Endothelial Growth Factor A - metabolism
Vascular Endothelial Growth Factor A - pharmacology
VEGF
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Summary:Attachment of growth factors to biodegradable polymers, such as poly(lactide-co-glycolide) (PLGA), may enhance and/or accelerate integration of tissue engineering scaffolds. Although proteins are commonly bound via abundant amino groups, a more selective approach may increase bioactivity of immobilized molecules. In this research, exposed carboxyl groups on acid-terminated PLGA were modified with dihydrazide spacer molecules. The number of hydrazide groups available for subsequent attachment of protein was dependent on dihydrazide length, with shorter molecules present at significantly greater surface densities. The potent angiogenic glycoprotein vascular endothelial growth factor (VEGF) was oxidized with periodate and the aldehyde moieties allowed to react with the hydrazide-derivatized PLGA. Derivatization initially affected the amount of protein bound to the surfaces, but differences were substantially reduced following overnight incubation in saline. More importantly, use of shorter dihydrazide spacers significantly enhanced accessibility of immobilized VEGF for binding neutralizing antibody and soluble VEGF receptor. Furthermore, immobilized growth factor enhanced endothelial cell proliferation, with surfaces having the shortest and longest spacers stimulating greater effects. The present work has not only demonstrated an alternative approach to immobilizing growth factors on biodegradable materials, but the scheme can be used to alter the amount of protein bound as well as its availability for subsequent biointeractions.
ISSN:1742-7061
1878-7568
DOI:10.1016/j.actbio.2008.02.017