Clinical, genetic and microbiological findings in a Brazilian family with aggressive periodontitis

Background, aim: Aggressive periodontitis comprises a group of rapidly progressive forms of periodontitis. Besides bacteria, a high level of subject susceptibility must be involved in the expression of disease. In the present study, we report the clinical, microbiological and genetic profile of a 14...

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Published in:Journal of clinical periodontology 2002-03, Vol.29 (3), p.233-239
Main Authors: Trevilatto, Paula C., Tramontina, Vinícius A., Machado, Maria A. N., Gonçalves, Reginaldo B., Sallum, Antonio W., Line, Sergio R. P.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aggregatibacter actinomycetemcomitans - isolation & purification
aggressive periodontitis
Alleles
Bacteroides - isolation & purification
Brazil
Child
cytokine
Dentistry
Female
gene polymorphism
Genetic Predisposition to Disease
Humans
Interleukin-1 - genetics
Male
Middle Aged
Pedigree
Periodontitis - genetics
Periodontitis - microbiology
Periodontitis - pathology
periopathogens
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Porphyromonas gingivalis - isolation & purification
Receptors, Interleukin-1 - antagonists & inhibitors
Risk Factors
Treponema - isolation & purification
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Summary:Background, aim: Aggressive periodontitis comprises a group of rapidly progressive forms of periodontitis. Besides bacteria, a high level of subject susceptibility must be involved in the expression of disease. In the present study, we report the clinical, microbiological and genetic profile of a 14‐individual family with aggressive periodontitis. Method: PCR was utilized to detect pathogenic bacteria of affected sites. DNA was obtained from epithelial cells through a mouthwash with 3% glucose and scrapping of the oral mucosa. RFLP‐PCR was used to analyze cytokine genetic polymorphisms. Results: Localized aggressive periodontitis was diagnosed for an 18‐year‐old systemically healthy non‐smoking proband, with siblings displaying aggressive periodontitis. Bacteroides forsythus and Treponema denticola were the most frequent pathogens. The proband presented Actinobacillus actinomycetemcomitans and detectable levels of Porphyromonas gingivalis, Bacteroides forsythus and Treponema denticola. Allele 2 of IL‐1α (−889) polymorphism was found in all individuals as well as allele 1 of the IL‐1β (+3953) gene. Alleles 1 and 2 (50 % each) of IL‐1β (−511), allele 1 of TNF‐α (−308) and allele 2 (in homo or heterozygosity) of IL‐RN (intron 2) gene were present. Conclusion: The results show that the present microbiological and genetic parameters were not relevant for the prediction of periodontitis susceptibility in this family. Zusammenfassung Zielsetzung: Unter dem Begriff aggressive Parodontitis wird eine Gruppe rasch fortschreitender Formen von Parodontitis zusammengefasst. Außer Bakterien muss ein hoher Grad individueller Suszeptibilität vorhanden sein, damit die Erkrankung zum Ausbruch kommt. In dieser Studie wird über das klinische, genetische und mikrobiologische Profil einer Familie von 14 Personen berichtet, in der aggressive Parodontitis auftrat. Material und Methoden: Mittels Polymerase‐Kettenreaktion (PCR) wurden parodontalpathogene Keime (Porphyromonas gingivalis, Porphyromonas endodontalis, Bacteroides forsythus, Actinobacillus actinomycetemcomitans, Prevotella intermedia, Prevotella nigrescens und Treponema denticola) aus Plaqueproben der erkrankten Stellen identifiziert. DNS wurde aus oralen Epithelzellen durch Mundspülung mit 3%iger Glukoselösung und Abwischen der Mundschleimhaut gewonnen. Die PCR‐Technik wurde auch genutzt, um genetische Zytokinpolymorphismen zu untersuchen. Ergebnisse: 6 Geschwister zeigten das klinische Bild einer aggressiven Parodontit
ISSN:0303-6979
1600-051X
DOI:10.1034/j.1600-051x.2002.290309.x